Intranasal immunization of lambs with serine/threonine phosphatase 2A against gastrointestinal nematodes
| Autor: | María Elena González-Sánchez, María Jesús Corral-Caridad, Mercedes Gomez Samblas, José Mª Alunda, Antonio Osuna, Elshaima Mohamed Fawzi, M. Cuquerella, Teresa Cruz Bustos, Gloria González-González, J. Solano, L.M. De Pablos |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Nematoda medicine.medical_treatment animal diseases Clinical Biochemistry Immunology Population Antibodies Helminth Helminthiasis Biology Microbiology Feces Serine-Threonine Phosphatase Immune system Adjuvants Immunologic Cell Wall parasitic diseases medicine Immunology and Allergy Helminths Animals Protein Phosphatase 2 Intestinal Diseases Parasitic education Nematode Infections Parasite Egg Count Administration Intranasal education.field_of_study Vaccines Sheep Bacteria business.industry Body Weight Vaccination medicine.disease biology.organism_classification Teladorsagia circumcincta Recombinant Proteins Intestinal Diseases Immunization Nematode infection Antigens Helminth Nasal administration Female Erratum business Adjuvant Haemonchus contortus |
| Zdroj: | Clinical and vaccine immunology : CVI. 20(9) |
| ISSN: | 1556-679X |
| Popis: | Seven 3-month-old, female, helminth-free lambs were immunized intranasally with three doses (1 mg total) of a recombinant part of the catalytic region of the serine/threonine phosphatase 2A (PP2Ar) (group 1 [G1]). In addition, four lambs were used as an adjuvant control group (G2), four as unimmunized, infected controls (G3), and four as unimmunized, uninfected controls (G4). Fifteen days after the last immunization, lambs from G1, G2, and G3 were challenged with 10,000 larval stage 3 (L3) organisms in a plurispecific nematode infection composed of ca. 40% Trichostrongylus colubriformis , 40% Haemonchus contortus , and 20% Teladorsagia circumcincta . All the lambs were clinically monitored throughout the experiment. Parasitological (fecal egg output and immunological response), biopathological (packed-cell volume and leukocyte and eosinophil counts), and zootechnical (live-weight gain) analyses were conducted. On day 105 of the experiment, all the animals were slaughtered and the adult worm population in their abomasa examined. Intranasal administration of PP2Ar with bacterial walls as an adjuvant elicited a strong immune response in the immunized lambs, as evidenced by their humoral immune response. Immunized animals and animals receiving the adjuvant shed significantly ( P < 0.001) fewer numbers of parasites' eggs in their feces. The immunization significantly reduced the helminth burden in the abomasa by the end of the experiment (>68%), protection being provided against both Haemonchus and Teladorsagia . Live-weight gain in the immunized lambs was similar to that in the uninfected controls versus the infected or adjuvanted animal groups. Our results suggest that heterologous immunization of ruminants by intranasal administration may be efficacious in the struggle to control gastrointestinal helminths in these livestock. |
| Databáze: | OpenAIRE |
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