Endosomolytic Activity of Cationic Liposomes Enhances the Delivery of Human Immunodeficiency Virus-1 Trans-activator Protein (TAT) to Mammalian Cells
| Autor: | Annette G. Teepe, Leaf Huang, Natalya V. Serbina, James Barsoum, Hassan Farhood |
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| Rok vydání: | 1995 |
| Předmět: |
Cell
Biophysics Endosomes Biology Endocytosis Biochemistry chemistry.chemical_compound Drug Delivery Systems Cations Tumor Cells Cultured medicine Humans Cationic liposome Molecular Biology Phosphatidylethanolamine Liposome Reporter gene Cell Biology Transfection Molecular biology medicine.anatomical_structure chemistry Epidermoid carcinoma Gene Products tat Liposomes HIV-1 tat Gene Products Human Immunodeficiency Virus lipids (amino acids peptides and proteins) |
| Zdroj: | Biochemical and Biophysical Research Communications. 217:761-768 |
| ISSN: | 0006-291X |
| Popis: | We have explored the use of cationic liposomes to deliver the human immunodeficiency virus-1 trans-activator protein tat using a reporter gene expression assay. The human epidermoid carcinoma cell A431 stably transfected with a reporter gene under the control of human immunodeficiency virus-1 promoter was used as a target cell. Phosphatidylcholine-containing cationic liposomes had no detectable tat delivery activity. In contrast, delivery of tat was enhanced by up to 150-fold using cationic liposomes enriched with dioleoyl phosphatidylethanolamine (DOPE), a lipid which readily transforms a bilayer into a nonbilayer structure. Enhanced delivery of tat by DOPE-containing liposomes was most likely the result of the endosomolytic activity of the liposome. This phospholipid-rich formulation showed no toxicity at concentrations sufficient for maximal delivery of tat. A variety of cationic liposome formulations which contain DOPE were tested successfully for tat delivery. |
| Databáze: | OpenAIRE |
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