SARS-CoV-2 receptors and entry genes are expressed in the human olfactory neuroepithelium and brain
Autor: | Basile Nicolas Landis, Joël Tuberosa, Ivan Rodriguez, Kristof Egervari, Alan Carleton, Daniel Rossier, Veronique Pauli, Johannes Alexander Lobrinus, Leon Fodoulian, Chenda Kan, Madlaina Boillat |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Bioinformatics media_common.quotation_subject Anosmia Omics Sensory system 02 engineering and technology ddc:616.07 Biology Microbiology Virus Article 03 medical and health sciences ddc:590 Virology medicine lcsh:Science Internalization Receptor Enhancer media_common Multidisciplinary Virus receptor Cell Biology Biological Sciences 021001 nanoscience & nanotechnology ddc:616.8 Olfactory bulb Cell biology 030104 developmental biology lcsh:Q medicine.symptom 0210 nano-technology hormones hormone substitutes and hormone antagonists |
Zdroj: | iScience iScience, Vol 23, Iss 12, Pp 101839-(2020) iScience, Vol. 23, No 12 (2020) P. 101839 |
ISSN: | 2589-0042 |
Popis: | Reports indicate an association between COVID-19 and anosmia, as well as the presence of SARS-CoV-2 virions in the olfactory bulb. To test whether the olfactory neuroepithelium may represent a target of the virus, we generated RNA-seq libraries from human olfactory neuroepithelia, in which we found substantial expression of the genes coding for the virus receptor angiotensin-converting enzyme-2 (ACE2), and for the virus internalization enhancer TMPRSS2. We analyzed a human olfactory single-cell RNA-seq dataset and determined that sustentacular cells, which maintain the integrity of olfactory sensory neurons, express ACE2 and TMPRSS2. ACE2 protein was highly expressed in a subset of sustentacular cells in human and mouse olfactory tissues. Finally, we found ACE2 transcripts in specific brain cell types, both in mice and humans. Sustentacular cells thus represent a potential entry door for SARS-CoV-2 in a neuronal sensory system that is in direct connection with the brain. Graphical Abstract Highlights • SARS-CoV-2 receptors ACE2 and TMPRSS2 are expressed in olfactory neuroepithelia • ACE2 and TMPRSS2 are coexpressed in supporting sustentacular cells • A subset of neuronal and non-neuronal cells in the brain transcribe ACE2 |
Databáze: | OpenAIRE |
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