Glucosensing capacity of rainbow trout telencephalon
| Autor: | Rosa Álvarez-Otero, Jesús M. Míguez, Ana Rocha, Rosa M. Ceinos, José Miguel Cerdá-Reverter, Cristina Otero-Rodiño, Marcos A. López-Patiño, José L. Soengas |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Cerdá-Reverter, José Miguel [0000-0003-1405-5750], Soengas, José L. [0000-0002-6847-3993], Cerdá-Reverter, José Miguel, Soengas, José L. |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Telencephalon medicine.medical_specialty Pro-Opiomelanocortin Endocrinology Diabetes and Metabolism Neuropeptide AMP-Activated Protein Kinases Deoxyglucose CREB 03 medical and health sciences Cellular and Molecular Neuroscience Sodium-Glucose Transporter 1 0302 clinical medicine Endocrinology Internal medicine Glucokinase medicine Animals Neuropeptide Y Phosphorylation Protein kinase A Mechanistic target of rapamycin Protein kinase B Liver X Receptors biology Endocrine and Autonomic Systems Chemistry AMPK Neuropeptide Y receptor Mitochondria Glucose 030104 developmental biology Rainbow trout Oncorhynchus mykiss biology.protein 030217 neurology & neurosurgery Glucosensing Signal Transduction |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | To assess the hypothesis of glucosensing systems present in fish telencephalon, we first demonstrated in rainbow trout, by in situ hybridisation, the presence of glucokinase (GK). Then, we assessed the response of glucosensing markers in rainbow trout telencephalon 6 hours after i.c.v. treatment with glucose or 2‐deoxyglucose (inducing glucoprivation). We evaluated the response of parameters related to the mechanisms dependent on GK, liver X receptor (LXR), mitochondrial activity, sweet taste receptor and sodium‐glucose linked transporter 1 (SGLT‐1). We also assessed mRNA abundance of neuropeptides involved in the metabolic control of food intake (agouti‐related protein, neuropeptide Y, pro‐opiomelanocortin, and cocaine‐ and amphetamine‐related transcript), as well as the abundance and phosphorylation status of proteins possibly involved in linking glucosensing with neuropeptide expression, such as protein kinase B (AkT), AMP‐activated protein kinase (AMPK), mechanistic target of rapamycin and cAMP response element‐binding protein (CREB). The responses obtained support the presence in the telencephalon of a glucosensing mechanism based on GK and maybe one based on LXR, although they do not support the presence of mechanisms dependent on mitochondrial activity and SGLT‐1. The mechanism based on sweet taste receptor responded to glucose but in a converse way to that characterised previously in the hypothalamus. In general, systems responded only to glucose but not to glucoprivation. Neuropeptides did not respond to glucose or glucoprivation. By contrast, the presence of glucose activates Akt and inhibits AMPK, CREB and forkhead box01. This is the first study in any vertebrate species in which the response to glucose of putative glucosensing mechanisms is demonstrated in the telencephalon. Their role might relate to processes other than homeostatic control of food intake, such as the hedonic and reward system Spanish Agencia Estatal de Investigacion and FEDER, Grant/Award Number: AGL2016-74857-C3-1-R, AGL2016-74857-C3-3-R and BES -2014 -068040; the European Fund of Regional Development |
| Databáze: | OpenAIRE |
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