Changes in Collagen Turnover in Early Acute Respiratory Distress Syndrome
| Autor: | A R Poole, Ann B. Millar, L Armstrong, David R Thickett, J P Mansell, M Ionescu, E Hoyle, R C Billinghurst |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine medicine.medical_specialty Pathology ARDS Adolescent Enzyme-Linked Immunosorbent Assay Lung injury Critical Care and Intensive Care Medicine Gastroenterology Risk Factors Internal medicine Pulmonary fibrosis Humans Urea Medicine Aged Aged 80 and over Respiratory Distress Syndrome Lung medicine.diagnostic_test business.industry Respiratory disease Middle Aged medicine.disease Procollagen peptidase Bronchoalveolar lavage medicine.anatomical_structure Female Collagen business Bronchoalveolar Lavage Fluid Oligopeptides Procollagen Type I collagen |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/ajrccm.160.6.9811084 |
| Popis: | Pulmonary fibrosis is a well-recognized feature of acute respiratory distress syndrome (ARDS). Using immunoassays of bronchoalveolar lavage (BAL), fluid we investigated the synthesis of type I procollagen (PICP) and type I/II collagen degradation products (COL2-3/4C(short) neoepitope) in patients with ARDS, acute lung injury (ALI), subjects with risk factors for ARDS (At Risk), and healthy/ventilated control subjects. PICP was measured by ELISA as a marker of type I procollagen synthesis. COL2-3/4C(short) neoepitope was measured by an inhibition ELISA as a marker of collagenase degradation of type I/II collagen. BAL was performed initially within 48 h of ventilation (Day 1) and then subsequently on Day 4. Dilution of epithelial lining fluid (ELF) was corrected for by plasma urea comparison. Increased PICP levels were observed in the ELF from ARDS and ALI subjects on Day 1 compared with subjects At Risk (median values, 124.9 and 95.0 ng/ml versus 38.0 ng/ml, respectively, p < 0.0005). By contrast, the levels of COL2-3/4C(short) neoepitope were significantly reduced in the subjects with ARDS versus the At Risk subjects (13.22 ng/ml versus 32.33 ng/ml, p < 0.0005). This translated into a greatly increased PICP:COL2-3/4C(short) ratio in the subjects with ARDS (p < 0.0001). There was a significant decline in the PICP level in the subjects with ARDS between Days 1 and 4 (n = 15, p < 0.05). Linear regression analysis showed a significant association between PICP and lung injury score in the subjects with ARDS (p = 0.01). Our data suggests an early shift in balance between type I collagen synthesis and degradation by collagenase. The resultant increase in type I collagen would favor matrix deposition and the development of pulmonary fibrosis in the lungs of subjects with ARDS. |
| Databáze: | OpenAIRE |
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