Receptor Usage of a Novel Bat Lineage C Betacoronavirus Reveals Evolution of Middle East Respiratory Syndrome-Related Coronavirus Spike Proteins for Human Dipeptidyl Peptidase 4 Binding

Autor: Syed Shakeel Ahmed, Wenhui Li, Kenneth S. M. Li, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Yinyan Sun, Antonio C.P. Wong, Dong-Yan Jin, Susanna K. P. Lau, Pyrear S.H. Zhao, Xiangyang He, Libiao Zhang, Patrick C. Y. Woo, Jian Piao Cai, Lifeng Xiong, Hayes K.H. Luk, Rachel Y.Y. Fan, Honglin Chen, Xingwen Peng, Terrence Chi-Kong Lau, Raven K. H. Kok
Přispěvatelé: Plazi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
viruses
Hypsugo bat
medicine.disease_cause
Chiroptera
Receptors
Immunology and Allergy
Viridae
Receptor
spike glycoprotein
Phylogeny
Coronavirus
Genetics
biotic associations
corona viruses
virus diseases
covid
Virus
Infectious Diseases
covid-19
Viruses
Spike Glycoprotein
Coronavirus
Receptors
Virus
Sequence Analysis
Binding domain
CETAF-taskforce
Protein Binding
Lineage (genetic)
animal structures
Middle East respiratory syndrome coronavirus
Evolution
Coronaviridae
Dipeptidyl Peptidase 4
Biology
virus-host
Evolution
Molecular
03 medical and health sciences
Major Articles and Brief Reports
Betacoronavirus
pathogen-host
medicine
Animals
Humans
biotic relations
Dipeptidyl peptidase-4
Molecular
pathogens
DNA
Sequence Analysis
DNA
biochemical phenomena
metabolism
and nutrition
Virus Internalization
medicine.disease
biology.organism_classification
biotic interaction
respiratory tract diseases
030104 developmental biology
HEK293 Cells
Middle East respiratory syndrome
Middle East Respiratory Syndrome coronavirus
Zdroj: The Journal of Infectious Diseases
ISSN: 1537-6613
0022-1899
Popis: The discovery of Hp-BatCoV HKU25 bridges the evolutionary gap between MERS-CoV and existing bat viruses, and suggests that bat viruses may have evolved to generate MERS-CoV through modulation of the spike protein for binding to hDPP4.
Although bats are known to harbor Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses, the role of bats in the evolutionary origin and pathway remains obscure. We identified a novel MERS-CoV-related betacoronavirus, Hp-BatCoV HKU25, from Chinese pipistrelle bats. Although it is closely related to MERS-CoV in most genome regions, its spike protein occupies a phylogenetic position between that of Ty-BatCoV HKU4 and Pi-BatCoV HKU5. Because Ty-BatCoV HKU4 but not Pi-BatCoV HKU5 can use the MERS-CoV receptor human dipeptidyl peptidase 4 (hDPP4) for cell entry, we tested the ability of Hp-BatCoV HKU25 to bind and use hDPP4. The HKU25-receptor binding domain (RBD) can bind to hDPP4 protein and hDPP4-expressing cells, but it does so with lower efficiency than that of MERS-RBD. Pseudovirus assays showed that HKU25-spike can use hDPP4 for entry to hDPP4-expressing cells, although with lower efficiency than that of MERS-spike and HKU4-spike. Our findings support a bat origin of MERS-CoV and suggest that bat CoV spike proteins may have evolved in a stepwise manner for binding to hDPP4.
Databáze: OpenAIRE
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