Phosphorylation changes of CaMKII, ERK1/2, PKB/Akt kinases and CREB activation during early long-term potentiation at Schaffer collateral-CA1 mouse hippocampal synapses
| Autor: | Virginia Tancredi, Daniela Merlo, Cristiana Mollinari, Alessio Cardinale, Margherita D'Antuono, Giovanna De Chiara, Mauro Racaniello |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Long-Term Potentiation CREB Biochemistry Hippocampus Settore BIO/09 Cellular and Molecular Neuroscience Mice Protein phosphorylation Internal medicine Ca2+/calmodulin-dependent protein kinase medicine LTP induction Serine Animals Phosphorylation Cyclic AMP Response Element-Binding Protein Protein kinase B Early Growth Response Protein 1 Mitogen-Activated Protein Kinase 1 Mice Inbred BALB C Mitogen-Activated Protein Kinase 3 Early-long term potentiation Mouse hippocampal slices Gene expression biology Brain-Derived Neurotrophic Factor musculoskeletal neural and ocular physiology Genes fos Long-term potentiation General Medicine Electric Stimulation Endocrinology medicine.anatomical_structure nervous system Schaffer collateral Synapses biology.protein Calcium-Calmodulin-Dependent Protein Kinase Type 2 Proto-Oncogene Proteins c-akt |
| Zdroj: | Neurochemical research 35 (2010): 239–246. doi:10.1007/s11064-009-0047-0 info:cnr-pdr/source/autori:Racaniello M.1,2; Cardinale A.1; Mollinari C.3,4; D'Antuono M.2; De Chiara G.3; Tancredi V.2; Merlo D.1,3/titolo:Phosphorylation changes of CaMKII, ERK1%2F2, PKB%2FAkt kinases and CREB activation during early long-term potentiation at Schaffer collateral-CA1 mouse hippocampal synapses/doi:10.1007%2Fs11064-009-0047-0/rivista:Neurochemical research/anno:2010/pagina_da:239/pagina_a:246/intervallo_pagine:239–246/volume:35 |
| Popis: | Protein phosphorylation is the main signaling system known to trigger synaptic changes underlying long-term potentiation (LTP). The timing of these phosphorylations plays an essential role to maintain the potentiated state of synapses. However, in mice a simultaneous analysis of phosphorylated proteins during early-LTP (E-LTP) has not been thoroughly carried out. Here we described phosphorylation changes of alphaCaMKII, ERK1/2, PKB/Akt and CREB at different times after E-LTP induced at Schaffer collateral/commissural fiber-CA1 synapses by 1 s 100 Hz tetanic stimulation in mouse hippocampal slices. We found that phosphorylation levels of all the molecules examined rapidly increased after tetanisation and remained above the basal level up to 30 min. Notably, we observed a sustained increment in the phosphorylation level of Akt at Ser473, whereas the phosphorylation level of Akt at Thr308 was unchanged. Unexpectedly, we also detected a marked increase of CREB target genes expression levels, c-fos, Egr-1 and exon-III containing BDNF transcripts. Our findings, besides providing a detailed timing of phosphorylation of the major kinases involved in E-LTP in mice, revealed that a modest LTP induction paradigm specifically triggers CREB-mediated gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|