Metabolic responses of sulfatide and related glycolipids in Madin–Darby canine kidney (MDCK) cells under osmotic stresses
| Autor: | Yukio Niimura, Ken-ichi Nagai |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Ceramide
Physiology Sodium chemistry.chemical_element Sodium Chloride Kidney Biochemistry Gene Expression Regulation Enzymologic Cell Line chemistry.chemical_compound Dogs Glycolipid Hypotonic Stress Osmotic Pressure Animals RNA Messenger Molecular Biology Messenger RNA Sulfoglycosphingolipids Osmotic concentration Chemistry Lipid Metabolism Molecular biology Enzyme Activation N-Acylsphingosine Galactosyltransferase Hypertonic Stress Tonicity Sulfotransferases |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 149:161-167 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/j.cbpb.2007.09.002 |
| Popis: | Incorporation of (35)S-sulfate into the polar molecular species of sulfoglycolipids (SM4s) in Madin-Darby canine kidney cells increased in a hypertonic medium (500 mOsm/L) supplemented with sodium chloride. The unknown sulfoglycolipid (SX) was identified as GlcCer sulfate based on the results of TLC, GLC, and mass spectra. The synthesis of SX increased in the hypotonic medium unlike that of SM4s and SM3. TLC showed that hypertonic stress induced the accumulation of GalCer as a precursor of SM4s, whereas hypotonic stress increased GlcCer as a precursor of GlcCer sulfate. The level of ceramide as a precursor of both GalCer and GlcCer increased under hypertonic stress and decreased under hypotonic stress. Cerebroside sulfotransferase mRNA was shown to be elevated in the hyperosmotic condition but not in the hypotonic condition. The increase in SM4s under hypertonic stress was induced by the activation of both the ceramide galactosyltransferase and the cerebroside sulfotransferase genes, whereas the increase in GlcCer sulfate under hypotonic stress was caused by the accumulation of GlcCer as the result of activation of ceramide glucosyltransferase. |
| Databáze: | OpenAIRE |
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