Time course of vitamin C distribution and absorption after oral administration in SMP30/GNL knockout mice
| Autor: | Kentaro Shimokado, Akihito Ishigami, Mizuki Iwama, Naoki Maruyama |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Ratón Endocrinology Diabetes and Metabolism Administration Oral White adipose tissue Ascorbic Acid Mice Oral administration In vivo Internal medicine Blood plasma medicine Animals Tissue Distribution Mice Knockout Nutrition and Dietetics Vitamin C Chemistry Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins Ascorbic acid Endocrinology Intestinal Absorption Knockout mouse Ascorbic Acid Deficiency Carboxylic Ester Hydrolases |
| Zdroj: | Nutrition (Burbank, Los Angeles County, Calif.). 27(4) |
| ISSN: | 1873-1244 |
| Popis: | Objective: Because vitamin C (VC) has multiple metabolic and antioxidant functions, we investigated the movement of VC throughout the tissues of senescence marker protein-30 (SMP30)/ gluconolactonase (GNL) knockout (KO) mice. Methods: SMP30/GNL KO mice, which cannot synthesize VC in vivo, were divided into two groups: VC sufficient and VC deficient. Starting at 2 mo of age, both groups had free access to water containing 1.5 and 0.0375 g/L of VC for 1 mo. Results: The average rate of VC retention in 20 tissues of VC-deficient SMP30/GNL KO mice was only 13.7% of that in VC-sufficient mice. Tissues that retained over 20% of VC were the cerebellum, white fat, testes, eyeballs, and pancreas, and those with less than 5% VC were the kidneys and heart. These results clearly indicate the different VC retention capacities among tissues. Next, we examined the time course of VC distribution and absorption in VC-deficient SMP30/GNL KO mice. After oral VC administration, VC content in the liver and kidney peaked at 3 h and then decreased. VC content in the lungs, adrenal glands, skin, white fat, and pancreas peaked at 6 h and in the cerebellum, cerebrum, skeletal muscles, eyeballs, thyroid gland, and testes at 12 h. Conclusion: In this study, we found that exogenous VC administered orally in VC-deficient SMP30/ GNL KO mice was distributed at distinctly different rates within individual tissues. The SMP30/GNL KO mice used in this study are a useful animal model that provides unique opportunities for investigating VC movement and metabolism in the entire body. |
| Databáze: | OpenAIRE |
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