Insulin-like growth factor (IGF)-I and IGF-II stimulate progesterone production by human luteal cells: role of IGF-I as mediator of growth hormone action
| Autor: | Fiorella Miceli, Alessandro Caruso, Nicoletta Di Simone, Daniela de Feo, Rosanna Apa, Salvatore Mancuso, E. Ronsisvalle, Antonio Lanzone |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
endocrine system medicine.medical_specialty medicine.medical_treatment Ovary Biology Luteal phase Chorionic Gonadotropin Insulin-like growth factor Basal (phylogenetics) Mediator Insulin-Like Growth Factor II Luteal Cells Internal medicine medicine Humans Insulin-Like Growth Factor I Cells Cultured Progesterone reproductive and urinary physiology Dose-Response Relationship Drug urogenital system Growth factor Obstetrics and Gynecology Drug Synergism Middle Aged IGF-I Settore MED/40 - GINECOLOGIA E OSTETRICIA medicine.anatomical_structure Endocrinology Reproductive Medicine Growth Hormone Insulin-like growth factor 2 biology.protein Female Corpus luteum hormones hormone substitutes and hormone antagonists |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)58445-3 |
| Popis: | Objective To examine the possible direct effect of insulin-like growth factor (IGF)-I and IGF-II on basal and hCG-stimulated P production by cultured human luteal cells. The possible role of IGF-I as mediator of GH action on luteal steroidogenesis also was investigated. Design Cultures of human luteal cells from early and midluteal phase. Setting All corpora lutea were obtained from the Obstetrics and Gynecology Department of the Universita Cattolica, a public care center. Patients Eight nonpregnant women between 35 and 47 years of age underwent surgery for various nonendocrine disorders such as leiomyomatosis. Interventions Corpora lutea were obtained at the time of hysterectomy. Main Outcome Measures Luteal cells were incubated with IGF-I or IGF-II with or without hCG at different concentrations. Growth hormone also was used alone and with an anti-IGF-I-antibody. Results We found that IGF-I and IGF-II were able to stimulate directly the P production at all used concentrations and that both of them significantly amplified the steroidogenic hCG effect. Finally, IGF-I was shown to mediate the positive GH action on P synthesis. |
| Databáze: | OpenAIRE |
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