Red Pepper Seed Inhibits Differentiation of 3T3-L1 Cells during the Early Phase of Adipogenesis via the Activation of AMPK
| Autor: | Hwa-Jin Kim, Ziyun Wang, Mi-Kyoung You, Hyeon-A Kim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene Expression AMP-Activated Protein Kinases Fatty Acid-Binding Proteins Fatty acid-binding protein Mice 03 medical and health sciences 3T3-L1 Cells Adipocytes Animals Phosphorylation Protein kinase A Receptor Adipogenesis biology Plant Extracts Chemistry Water AMPK Cell Differentiation General Medicine Peroxisome Lipid Metabolism Cell biology PPAR gamma Fatty acid synthase 030104 developmental biology Complementary and alternative medicine CCAAT-Enhancer-Binding Proteins biology.protein Capsicum Sterol Regulatory Element Binding Protein 1 |
| Zdroj: | The American Journal of Chinese Medicine. 46:107-118 |
| ISSN: | 1793-6853 0192-415X |
| DOI: | 10.1142/s0192415x18500064 |
| Popis: | Obesity is the main risk factor for metabolic syndromes and there has been an upsurge in demand for effective therapeutic strategies. This study investigated the effect of red pepper seed water extract (RPS) on the process of differentiation in 3T3-L1 adipocytes. RPS treatment significantly suppressed cellular lipid accumulation and reduced the expression of adipocytes-associated proteins, peroxisome proliferator-activated receptor-[Formula: see text] (PPAR-[Formula: see text]), CCAAT/enhancer-binding proteins [Formula: see text] (C/EBP [Formula: see text]), sterol regulatory element binding protein-1c (SREBP-1c), as well as fatty acid synthase (FAS), and fatty acid binding protein 4 (FABP4). The inhibitory effect of RPS on differentiation was mainly through the modulation of the C/EBP [Formula: see text] and C/EBP [Formula: see text] expression at the early phase of differentiation. Moreover, at the early phase of differentiation, RPS markedly increased the phosphorylation of AMP-activated protein kinase (AMPK). Such enhancing effect of RPS was abolished in the presence of compound C. Our results suggest that activation of AMPK at early stage of adipogenesis is involved in the anti-adipogenesis effect of RPS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|