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Over the last 20 years specific membrane polypeptides, such as receptors, enzymes, and organelle-specific antigens, have been visualized in the electron microscope with 'pre-embedding' or 'postembedding' methods of tissue processing (Anderson et al., 1978; Louvard et al., 1982; Tokuyasu, 1986; Brown and Farquhar, 1989; Gonatas, J., et al., 1989; Roth, 1989; Chen et al., 1989). With 'pre-embedding' methods, tissue sections or cells are fixed and 'permeabilized' with a suitable detergent prior to the application of the labeling probe; the labeling probe is then visualized in the light and electron microscopes with horseradish peroxidase (HRP), which may be coupled directly to the labeling probe, or to a second ligand (Guesdon et al., 1979; Brown and Farquhar, 1989). The detection of HRP in the light and electron microscopes is then accomplished with the classic diaminobenzidine (DAB) reaction of Graham and Karnovsky (1966). In 'postembedding' immunocytochemistry, ceils or |
