A model of orthotopic murine bladder (MBT-2) tumor implants
| Autor: | X M Zhou, Feng Jiang |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Nephrology
medicine.medical_specialty Pathology Urology Biology Flow cytometry Mice Orthotopic transplantation Bladder cavity Internal medicine Submucosa Tumor Cells Cultured medicine Carcinoma Animals Mice Inbred C3H Bladder cancer medicine.diagnostic_test DNA Neoplasm Flow Cytometry medicine.disease Primary tumor Disease Models Animal Microscopy Electron medicine.anatomical_structure Urinary Bladder Neoplasms Neoplasm Transplantation |
| Zdroj: | Urological Research. 25:179-182 |
| ISSN: | 1434-0879 0300-5623 |
| DOI: | 10.1007/bf00941979 |
| Popis: | We produced a model of orthotopic transplantation in C3H/He mice by transplanting MBT-2 cells. A single-cell suspension of 1.0 x 10(5) MBT-2 cells/0.02 ml was successfully injected into the submucosal layer of the bladder, with an incidence of 100% after four experimental weeks. Inoculated tumor grew expansively into the bladder cavity from the bladder submucosa and invaded the serosa and the surrounding tissue. This model more closely resembled the characteristics of human bladder tumor when compared to other bladder cancer models. The results of the histological observation, electron microscopic examination and DNA content analysis by flow cytometry showed that the transplanted carcinoma maintained the biologic characteristics of the primary tumor. |
| Databáze: | OpenAIRE |
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