Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
| Autor: | Celeste Ruiz de Arcaute, Marcelo L. Larramendy, Noelia Nikoloff, Sonia Soloneski |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Imazethapyr-based formulation Imidazolinone herbicides COMET ASSAY DNA repair DNA damage Health Toxicology and Mutagenesis Otras Ciencias Biológicas 010501 environmental sciences Biology OXIDATIVE DNA DAMAGE medicine.disease_cause 01 natural sciences Ciencias Biológicas Deoxyribonuclease (Pyrimidine Dimer) 03 medical and health sciences chemistry.chemical_compound medicine Animals Environmental Chemistry Nucleoid Ciencias Naturales Comet assay 0105 earth and related environmental sciences Herbicides Escherichia coli Proteins Nicotinic Acids General Medicine IMAZETHAPYR-BASED FORMULATION Pollution Oxidative Stress 030104 developmental biology chemistry Biochemistry DNA glycosylase Oxidative DNA damage IMIDAZOLINONE HERBICIDES DNA Genotoxicity Oxidative stress CIENCIAS NATURALES Y EXACTAS DNA Damage |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| DOI: | 10.1007/s11356-017-8666-5 |
| Popis: | We evaluated the role of oxidative stress in the genotoxic damage induced by imazethapyr (IMZT) and its formulation Pivot® in mammalian CHO-K1 cell line. Using the alkaline comet assay, we observed that a concentration of 0.1 μg/mL of IMZT or Pivot® was able to induce DNA damage by increasing the frequency of damaged nucleoids. To test whether the DNA lesions were caused by oxidative stress, the DNA repair enzymes endonuclease III (Endo III) and formamidopyrimidine-DNA glycosylase (Fpg), which convert base damage to strand breaks, were used. Our results demonstrate that after treatment of CHO-K1 cells with the pure active ingredient as well as the commercial formulation Pivot®, an increase in DNA strand breaks was observed after incubation of both Endo III and Fpg enzymes, indicating that both compounds induce DNA damage involving both pyrimidine and purine-based oxidations, at least in CHO-K1 cells. Our findings confirm the genotoxic potential of IMZT and suggest that this herbicide formulation must be employed with great caution, especially not only for exposed occupational workers but also for other living species. Facultad de Ciencias Naturales y Museo |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|