Chromosomal alterations, biological features and in vitro chemosensitivity of SCLC-R1, a new cell line from human metastatic small cell lung carcinoma
| Autor: | Fulvia Farabegoli, A. Sensi, Wainer Zoli, Laura Roncuzzi, Dino Amadori, Anna Gasperi-Campani, Nicoletta Zini, R. Gruppioni, L Lenzi |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Antineoplastic Agents Biology Small-cell carcinoma Translocation Genetic Chromosome 16 FHIT Proto-Oncogene Proteins Biomarkers Tumor Tumor Cells Cultured medicine Humans Carcinoma Small Cell Lung cancer Chromosome 12 Aged Chromosome Aberrations DNA Neoplasm medicine.disease Diploidy Oncology Chromosome 3 Cell culture Cancer research Small Cell Lung Carcinoma Gene Deletion |
| Zdroj: | European Journal of Cancer. 34:724-730 |
| ISSN: | 0959-8049 |
| Popis: | A new human cancer cell line was established from a metastatic lesion of a small cell lung carcinoma (SCLC-R1) and maintained in continuous culture with a doubling time of 62 h. The SCLC-R1 line, whose ultrastructural features are presented, showed a diploid DNA content, a translocation involving chromosome 16 [t(16;?)(q24;?)] and noticeable deletions in the FHIT (fragile histidine triad) region in the short arm of chromosome 3 [del(3)(p14)] and in the telomeric region of the short arm of chromosome 12 [del(12)(p13)]. The involvement of 12p in metastatic small cell lung cancer is reported here for the first time. No amplification or rearrangements were evident in the c-myc, L-myc, N-myc, int-2, c-erbB-2, H-ras, K-ras, c-mos, and hst-1 genes by Southern blot analysis. Wild-type p53, RB, K-ras and H-ras genes were evident by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. The neuron specific enolase (NSE) level was much higher in the cell line's cytosol than in the patient's serum and the cell line also had high expression of chromogranin A and cytokeratin 19. SCLC-R1 cells were sensitive to cisplatin, carboplatin and doxorubicin. The clinical history of the patient from whom the cell line was derived is reported. The characteristics of this new cell line indicate it to be a useful experimental model to investigate lung cancer biology and anticancer drug response. |
| Databáze: | OpenAIRE |
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