Enrichment of Polyglucosylated Isoflavones from Soybean Isoflavone Aglycones Using Optimized Amylosucrase Transglycosylation

Autor: Cheon-Seok Park, Davin Jang, Young Sung Jung, Tae Gyu Nam, Mi-Seon Kim, Chan-Su Rha, Dae-Ok Kim, Ye-Jin Kim, Aaron Taehwan Kim, Dong-Ho Seo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
Glycosylation
beta-Glucans
animal diseases
Pharmaceutical Science
Genistein
01 natural sciences
glycosyltransferase
Mass Spectrometry
Analytical Chemistry
deinococcus geothermalis
chemistry.chemical_compound
hplc/ms
Drug Discovery
Food science
Deinococcus geothermalis
Chromatography
High Pressure Liquid
biology
virus diseases
food and beverages
Isoflavones
Glucosyltransferases
Chemistry (miscellaneous)
isoflavone α-glucoside
Molecular Medicine
Deinococcus
glycine max (l.) merr
isoflavone aglycone-rich extract
Genetic Vectors
Biological Availability
Phytoestrogens
Article
lcsh:QD241-441
Amylosucrase
lcsh:Organic chemistry
010608 biotechnology
parasitic diseases
Escherichia coli
Physical and Theoretical Chemistry
Plant Extracts
010405 organic chemistry
Organic Chemistry
Daidzein
Glycitein
biology.organism_classification
bacterial infections and mycoses
0104 chemical sciences
Aglycone
Glycine max (L.) Merr
chemistry
biology.protein
Soybeans
Zdroj: Molecules, Vol 25, Iss 1, p 181 (2020)
Molecules
Volume 25
Issue 1
ISSN: 1420-3049
Popis: Isoflavones in soybeans are well-known phytoestrogens. Soy isoflavones present in conjugated forms are converted to aglycone forms during processing and storage. Isoflavone aglycones (IFAs) of soybeans in human diets have poor solubility in water, resulting in low bioavailability and bioactivity. Enzyme-mediated glycosylation is an efficient and environmentally friendly way to modify the physicochemical properties of soy IFAs. In this study, we determined the optimal reaction conditions for Deinococcus geothermalis amylosucrase-mediated α-1,4 glycosylation of IFA-rich soybean extract to improve the bioaccessibility of IFAs. The conversion yields of soy IFAs were in decreasing order as follows: genistein >
daidzein >
glycitein. An enzyme quantity of 5 U and donor:acceptor ratios of 1000:1 (glycitein) and 400:1 (daidzein and genistein) resulted in high conversion yield (average 95.7%). These optimal reaction conditions for transglycosylation can be used to obtain transglycosylated IFA-rich functional ingredients from soybeans.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje