Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant mitosis and meiosis
| Autor: | M. Rosa, Wanda Viegas, Ana D. Caperta, R. Karimi, Margarida Delgado, Dmitri Demidov, Andreas Houben |
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| Rok vydání: | 2008 |
| Předmět: |
Threonine
Arabidopsis Mitosis Biology Chromosomes Plant histone phosphorylation Histones Histone H3 Species Specificity Meiosis Histone H1 Plant Cells Histone H2A Genetics meiosis Phosphorylation Molecular Biology Metaphase In Situ Hybridization Fluorescence Genetics (clinical) Plant Proteins Anaphase mitosis Secale Cell Cycle food and beverages Hordeum Plants Vicia faba Cell biology Histone phosphorylation Protein Processing Post-Translational |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000151319 |
| Popis: | Cell cycle dependent phosphorylation of conserved N-terminal tail residues of histone H3 has been described in both animal and plant cells. Through cytogenetic approaches using different plant species we show a detailed description of distribution patterns of phosphorylated histone H3 at either threonine 3 or threonine 32 in mitosis and meiosis. In meristematic cells of the large genome species Secale cereale, Vicia faba and Hordeum vulgare we have found that phosphorylation of both threonine residues begins in prophase, and dephosphorylation occurs in late anaphase. However, in the small genome species Arabidopsis thaliana dephosphorylation occurs at anaphase. In the first division of meiosis of species with large genomes phosphorylation of histone H3 at either threonine 3 or threonine 32 is seen first in diakinesis and extends to anaphase I, whereas in the second division these post-translational modifications are visible at metaphase II through anaphase II. While in A. thaliana dephosphorylation takes place at anaphase I and II. In all species analysed phosphorylated H3 at either threonine 3 or threonine 32 are distributed along the entire length of chromosomes during mitotic metaphase and metaphase I. In the second meiotic division threonine 3 phosphorylation is restricted to the pericentromeric domain, while phosphorylation of threonine 32 is widespread along chromosome arms of all species analysed. Research in the W.V. laboratory was supported by Fundação para a Ciência e Tecnologia, Portugal grant SFRH/BPD/26442 to A.D.C. and SFRH/ BPD/27219/2006 to M.D., respectively. D.D., R.K. and A.H. were supported by the Deutsche Forschungsgemeinschaft and by the Land Sachsen Anhalt |
| Databáze: | OpenAIRE |
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