Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland
| Autor: | Kirstin Frost, Barbara Druml, Karina Hettwer, Steffen Uhlig, Margit Cichna-Markl, Rupert Hochegger, Kirsten Simon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Health (social science)
Outlier removal Pcr assay detection TP1-1185 Plant Science Biology Health Professions (miscellaneous) Microbiology Article biology.animal parasitic diseases roe deer Food science Raw meat probability of detection validation Chemical technology Member states Repeatability food authentication quantification Roe deer interlaboratory ring trial Real-time polymerase chain reaction game meat real-time PCR Food Science |
| Zdroj: | Foods Foods, Vol 10, Iss 2645, p 2645 (2021) Volume 10 Issue 11 |
| ISSN: | 2304-8158 |
| Popis: | Game meat products are particularly prone to be adulterated by replacing game meat with cheaper meat species. Recently, we have presented a real-time polymerase chain reaction (PCR) assay for the identification and quantification of roe deer in food. Quantification of the roe deer content in % (w/w) was achieved relatively by subjecting the DNA isolates to a reference real-time PCR assay in addition to the real-time PCR assay for roe deer. Aiming at harmonizing analytical methods for food authentication across EU Member States, the real-time PCR assay for roe deer has been tested in an interlaboratory ring trial including 14 laboratories from Austria, Germany, and Switzerland. Participating laboratories obtained aliquots of DNA isolates from a meat mixture containing 24.8% (w/w) roe deer in pork, roe deer meat, and 12 meat samples whose roe deer content was not disclosed. Performance characteristics included amplification efficiency, level of detection (LOD95%), repeatability, reproducibility, and accuracy of quantitative results. With a relative reproducibility standard deviation ranging from 13.35 to 25.08% (after outlier removal) and recoveries ranging from 84.4 to 114.3%, the real-time PCR assay was found to be applicable for the detection and quantification of roe deer in raw meat samples to detect food adulteration. |
| Databáze: | OpenAIRE |
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