Identification of Naegleria fowleri proteins linked to primary amoebic meningoencephalitis
| Autor: | Francine Marciano-Cabral, Jay Park, Melissa Jamerson, Guy A. Cabral, Jacqueline A Schmoyer |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
food.ingredient 030106 microbiology Protozoan Proteins Virulence Central Nervous System Protozoal Infections Microbiology Amoeba (genus) Mice 03 medical and health sciences food Microscopy Electron Transmission Meningoencephalitis Serial passage parasitic diseases Cell Adhesion medicine Animals Flagellate Cytoskeleton Gene Naegleria fowleri biology Amebiasis medicine.disease biology.organism_classification Disease Models Animal 030104 developmental biology Microscopy Electron Scanning Signal Transduction |
| Zdroj: | Microbiology. 163:322-332 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.000428 |
| Popis: | Naegleria fowleri (N. fowleri) causes primary amoebic meningoencephalitis, a rapidly fatal disease of the central nervous system. N. fowleri can exist in cyst, flagellate or amoebic forms, depending on environmental conditions. The amoebic form can invade the brain following introduction into the nasal passages. When applied intranasally to a mouse model, cultured N. fowleri amoebae exhibit low virulence. However, upon serial passage in mouse brain, the amoebae acquire a highly virulent state. In the present study, a proteomics approach was applied to the identification of N. fowleri amoeba proteins whose expression was associated with the highly virulent state in mice. Mice were inoculated intranasally with axenically cultured amoebae or with mouse-passaged amoebae. Examination by light and electron microscopy revealed no morphological differences. However, mouse-passaged amoebae were more virulent in mice as indicated by exhibiting a two log10 titre decrease in median infective dose 50 (ID50). Scatter plot analysis of amoebic lysates revealed a subset of proteins, the expression of which was associated with highly virulent amoebae. MS-MS indicated that this subset contained proteins that shared homology with those linked to cytoskeletal rearrangement and the invasion process. Invasion assays were performed in the presence of a select inhibitor to expand on the findings. The collective results suggest that N. fowleri gene products linked to cytoskeletal rearrangement and invasion may be candidate targets in the management of primary amoebic meningoencephalitis. |
| Databáze: | OpenAIRE |
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