Visual and clinical analysis of Bac-T-Screen urine screen results
| Autor: | M B Tyburski, Ellen Jo Baron, Marjorie Berman, R Almon |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Pathology Bacteriuria Clinical pathology medicine.diagnostic_test Clinical Laboratory Techniques Cytodiagnosis Urinary system Urine Biology medicine.disease Staining law.invention Gram staining law Hemocytometer medicine Humans Research Article Urine cytology |
| Zdroj: | Journal of Clinical Microbiology. 26:2382-2386 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.26.11.2382-2386.1988 |
| Popis: | Of 337 urine specimens evaluated, 75 of the 113 that showed positive readings on the Bac-T-Screen urine-screening instrument were found by subsequent semiquantitative culture to yield either less than 10,000 CFU of mixed bacteria per ml or no growth (less than 100 CFU/ml by our criteria). We tried to determine what factors contributed to the positive Bac-T-Screen results by examining the 75 Bac-T-Screen-positive urine specimens with three visual methods: Gram staining, hemacytometer chamber counting, and filtering through a 5.0-microns-pore-size nitrocellulose filter with subsequent microscopic examination of the stained filter. Somatic cells and other particles present in those urine specimens that yielded positive readings by the Bac-T-Screen included epithelial cells in 43%, crystals and amorphous material in 33%, and leukocytes in 17% of the specimens. There was no relationship between the numbers of particles seen in urine and the magnitude of the relative absorbance reading obtained with the Bac-T-Screen. A retrospective chart review was conducted for patients with positive Bac-T-Screen results and negative cultures. Of the 75 patients, 6 were thought to have urinary tract infections on the basis of clinical criteria; the majority of the remaining 69 patients had clinical histories revealing systemic or urogenital conditions consistent with shedding of particles in the urine. A positive reading by the Bac-T-Screen system seemed to be related to the presence of somatic cells and other particles in urine; bacteriuria was not always detectable in these cases. |
| Databáze: | OpenAIRE |
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