Galactose utilization sheds new light on sugar metabolism in the sequenced strain Dekkera bruxellensis CBS 2499
| Autor: | Claudia Capusoni, Ileana Vigentini, Zhihao Ling, Silvia Galafassi, Jure Piškur, Moktaduzzaman, Concetta Compagno |
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| Rok vydání: | 2015 |
| Předmět: |
Nitrogen
Saccharomyces cerevisiae Brettanomyces Carbohydrate metabolism Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Fungal Ethanol fuel Gene Acetic Acid 030304 developmental biology 0303 health sciences Ethanol biology 030306 microbiology Galactose General Medicine Metabolism biology.organism_classification Carbon Yeast Glucose Biochemistry chemistry Crabtree effect Metabolic Networks and Pathways |
| Zdroj: | FEMS Yeast Research. 15 |
| ISSN: | 1567-1364 1567-1356 |
| DOI: | 10.1093/femsyr/fou009 |
| Popis: | Dekkera bruxellensis and Saccharomyces cerevisiae are considered two phylogenetically distant relatives, but they share several industrial relevant traits such as the ability to produce ethanol under aerobic conditions (Crabtree effect), high tolerance towards ethanol and acids, and ability to grow without oxygen. Beside a huge adaptability, D. bruxellensis exhibits a broader spectrum in utilization of carbon and nitrogen sources in comparison to S. cerevisiae. With the aim to better characterize its carbon source metabolism and regulation, the usage of galactose and the role that glucose plays on sugar metabolism were investigated in D. bruxellensis CBS 2499. The results indicate that in this yeast galactose is a non-fermentable carbon source, in contrast to S. cerevisiae that can ferment it. In particular, its metabolism is affected by the nitrogen source. Interestingly, D. bruxellensis CBS 2499 exhibits the 'short-term Crabtree effect', and the expression of genes involved in galactose utilization and in respiratory metabolism is repressed by glucose, similarly to what occurs in S. cerevisiae. |
| Databáze: | OpenAIRE |
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