Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by lipopolysaccharide
| Autor: | Hongwei Jiang, W. T. Qi, C. Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Lipopolysaccharide Cellular differentiation Blotting Western Osteoclasts Enzyme-Linked Immunosorbent Assay Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Multinucleate Animals Viability assay Dental papilla Dental Papilla General Dentistry Cell Proliferation Microscopy Confocal Chemistry Cell growth Cell Differentiation 030206 dentistry Molecular biology Resorption 030104 developmental biology Cell culture Odontogenesis Reactive Oxygen Species |
| Zdroj: | International Endodontic Journal. 51:e115-e124 |
| ISSN: | 0143-2885 |
| Popis: | Aim To investigate the role of Lipopolysaccharide (LPS) in the odontoclast differentiation of MDPC-23 cells. It was hypothesized that MDPC-23 odontoblast-like cells may function as odontoclasts under the influence of LPS. Methodology MDPC-23 cells were cultured in the presence of 0.1 or 1 μg mL−1 LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast-like function of the induced cells. In additional, the related protein expression was confirmed by Western blotting and ELISA. An unpaired Student's t-test and one-way anova were used in statistical analysis. Results TRAP-positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg mL−1 LPS-induced cells (P 0.05). ROS production was observed in the 1 μg mL−1 LPS treatment group (P 0.05). Conclusions A known value of 1 μg mL−1 LPS might induce odontoblast-like MDPC-23 cells to generate odontoclast-like cells or to function as odontoclasts. The data might provide a new explanation for the precursors of odontoclasts and root resorption. |
| Databáze: | OpenAIRE |
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