The alpha-7 nicotinic acetylcholine receptor is involved in a direct inhibitory effect of nicotine on GnRH release: In vitro studies
| Autor: | Elio Messi, Valentina Andre, Cecilia Gotti, Roberto Maggi, Chiara Rigobello, Federica Pimpinelli |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Nicotine alpha7 Nicotinic Acetylcholine Receptor Alpha (ethology) Gonadotropin-releasing hormone Biology Pharmacology Biochemistry Cell Line Gonadotropin-Releasing Hormone 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine medicine Cyclic AMP Humans Alprostadil Molecular Biology Depolarization Nicotinic acetylcholine receptor 030104 developmental biology Nicotinic agonist nervous system Potassium Cholinergic hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery Hormone medicine.drug |
| Zdroj: | Molecular and cellular endocrinology. 460 |
| ISSN: | 1872-8057 |
| Popis: | The activation of nicotinic cholinergic receptors (nAChR) inhibits the reproductive axis; however, it is not clear whether nicotine may directly modulate the release of hypothalamic gonadotropin-releasing hormone (GnRH). Experiments carried out in GT1-1 immortalized GnRH neurons reveal the presence of a single class of high affinity α4β2 and α7 nAchR subtypes. The exposure of GT1-1 cells to nicotine does not modify the basal accumulation of GnRH. However, nicotine was found to modify GnRH pulsatility in perifusion experiments and inhibits, the release of GnRH induced by prostaglandin E1 or by K+-induced cell depolarization; these effects were reversed by D-tubocurarine and α-bungarotoxin. In conclusion, the results reported here indicate that: functional nAChRs are present on GT1-1 cells, the activation of the α-bungarotoxin-sensitive subclass (α7) produces an inhibitory effect on the release of GnRH and that the direct action of nicotine on GnRH neurons may be involved in reducing fertility of smokers. |
| Databáze: | OpenAIRE |
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