Deletion of Yersinia pestis ail Causes Temperature-Sensitive Pleiotropic Effects, Including Cell Lysis, That Are Suppressed by Carbon Source, Cations, or Loss of Phospholipase A Activity
| Autor: | Anna M. Kolodziejek, Carolyn J. Hovde, Gregory A. Bohach, Scott A. Minnich |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lysis
LPS Yersinia pestis Virulence Factors Mutant Phospholipid Down-Regulation Sigma Factor Microbiology Lipid A chemistry.chemical_compound heat shock response Heat shock Molecular Biology phospholipid Bacterial Capsules Ail lysis thermosensitivity Growth medium membrane homeostasis biology Temperature Genetic Pleiotropy Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Carbon Glucose chemistry Phospholipases Calcium PldA Bacterial outer membrane Gene Deletion Research Article Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of Bacteriology |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Maintenance of phospholipid (PL) and lipopoly- or lipooligo-saccharide (LPS or LOS) asymmetry in the outer membrane (OM) of Gram-negative bacteria is essential but poorly understood. The Yersinia pestis OM Ail protein was required to maintain lipid homeostasis and cell integrity at elevated temperature (37° C). Loss of this protein had pleiotropic effects. A Y. pestis Δail mutant and KIM6+ wild- type were systematically compared for (i) growth requirements at 37° C, (ii) cell structure, (iii) antibiotic and detergent sensitivity, (iv) proteins released into supernates, (v) induction of the heat shock response, and (vi) physiological and genetic suppressors that restored the wild- type phenotype. The Δail mutant grew normally at 28° C but lysed at 37° C when it entered stationary phase as shown by cell count, SDS-PAGE of cell supernatants, and electron microscopy. Immuno-fluorescent microscopy showed that the Δail mutant did not assemble Caf1 capsule. Expression of heat shock promoters rpoE or rpoH fused to a lux operon reporter were not induced when the Δail mutant was shifted from the 28° C to 37° C (p |
| Databáze: | OpenAIRE |
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