Cruciferous phytoalexins: antiproliferative effects in T-Jurkat leukemic cells
| Autor: | Mojmír Suchý, P. Kutschy, Andrej Mirossay, Roman Mezencev, Zuzana Čurillová, Ján Mojžiš, Kenji Monde, Martina Pilátová, L. Mirossay, Marek Šarišský |
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| Rok vydání: | 2004 |
| Předmět: |
Cancer Research
Programmed cell death Cell cycle checkpoint Apoptosis Biology Jurkat cells chemistry.chemical_compound Jurkat Cells Annexin Phytoalexins medicine Humans Cell Death Dose-Response Relationship Drug Plant Extracts Terpenes Hematology medicine.disease Molecular biology Staining Leukemia Oncology chemistry Biochemistry Brassicaceae Sesquiterpenes DNA Phytotherapy |
| Zdroj: | Leukemia research. 29(4) |
| ISSN: | 0145-2126 |
| Popis: | We tested antiproliferative activity of selected cruciferous phytoalexins including brassinin, 1-methoxybrassinin, (+/-)-spirobrassin, (+/-)-1-methoxyspirobrassinin and (+/-)-1-methoxyspirobrassinol, in leukemic Jurkat cell. The most effective of the tested phytoalexins was 1-methoxybrassinin with IC(50) 10 micromol l(-1). However, significant effect of all phytoalexines was also determined at concentration 1 micromol l(-1). In 1-methoxybrassinin-treated Jurkat cells, we found significant increase in the fraction of cells with a sub-G(0)/G(1) DNA content, which is considered to be a marker of cell death by apoptosis. Apoptosis was also confirmed by the annexin V staining. In summary, 1-methoxybrassinin exerted potent antiproliferative activity probably due to cell cycle arrest and apoptosis induction. |
| Databáze: | OpenAIRE |
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