Functional assessment of cryopreserved human femoral arteries for pharmaceutical research
| Autor: | M.V. Martínez Santos, C. Andión Núñez, María Esther Rendal Vázquez, R.O. Fernández Mallo, G Matheu Capó, R Segura Iglesias, M. Rodríguez Cabarcos, S. Pértega Díaz, T Bermúdez González, P. Filgueira Fernández, J Sánchez Ibáñez |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_treatment Biomedical Engineering Vasodilation Femoral artery In Vitro Techniques Biology Cryopreservation Biomaterials Andrology Contractility Norepinephrine chemistry.chemical_compound Cryoprotective Agents medicine.artery medicine Humans Dimethyl Sulfoxide Saline Transplantation Dimethyl sulfoxide Cell Biology Middle Aged Acetylcholine Femoral Artery chemistry Vasoconstriction Anesthesia Female Endothelium Vascular Tissue Preservation Sodium nitroprusside medicine.symptom medicine.drug |
| Zdroj: | Cell and Tissue Banking. 5:105-110 |
| ISSN: | 1389-9333 |
| Popis: | An established method for cryopreservation that might preserve the vascular and endothelial responses of human femoral arteries (HFAs) to be transplanted as allografts was studied. HFAs were harvested from multiorgan donors and stored at 4 degrees C in saline solution before cryostorage. Thirty HFA rings were isolated and randomly assigned to one control group of unfrozen HFAs (eight rings) and one group of cryopreserved HFAs (22 rings). Cryopreservation was performed in RPMI solution containing dimethylsulfoxide (DMSO) and the rate of cooling was -1 degrees C/min until -40 degrees C and faster rates until -150 degrees C was reached. The contractile and relaxant responses of unfrozen and frozen/thawed arteries were assessed in organ bath by measurement of isometric force generated by the HFAs. After thawing, the maximal contractile responses to the contracting agonist tested (noradrenaline) were in the range of 43% of the responses in unfrozen HFAs. The endothelium-independent responses to sodium nitroprusside were not altered whereas the endothelium-dependent relaxant responses to acetylcholine were weakly altered. The cryopreservation method used provided a limited preservation of contractility of HFAs, a good preservation of the endothelium-independent relaxant responses, and a good preservation of endothelium-dependent relaxation. It is possible that further refinements of the cryopreservation protocol, such as a slower rate of cooling and a more controlled stepwise addition of DMSO, might allow better post-thaw functional recovery. |
| Databáze: | OpenAIRE |
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