DNA Methylation Profiles of Vegans and Non-Vegetarians in the Adventist Health Study-2 Cohort
| Autor: | Fayth L. Miles, Charles Wang, Kimberly D. Siegmund, Valery Filippov, Andrew Mashchak, Michael J. Orlich, Xin Chen, Gary E. Fraser, Penelope J. Duerksen-Hughes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine False discovery rate Diet Vegan vegetarian diet lcsh:TX341-641 Biology permutation White People Article Body Mass Index Cohort Studies 03 medical and health sciences 0302 clinical medicine Intergenic region Risk Factors Humans Epigenetics Gene Aged Vegans Genetics Nutrition and Dietetics DNA methylation epigenetics Diet Vegetarian Methylation Middle Aged Black or African American 030104 developmental biology CpG site 030220 oncology & carcinogenesis Cohort Linear Models linear regression Female Adventist Health Study-2 lcsh:Nutrition. Foods and food supply Vegetarians Food Science |
| Zdroj: | Nutrients Volume 12 Issue 12 Nutrients, Vol 12, Iss 3697, p 3697 (2020) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu12123697 |
| Popis: | We sought to determine if DNA methylation patterns differed between vegans and non-vegetarians in the Adventist Health Study-2 cohort. Genome-wide DNA methylation derived from buffy coat was profiled in 62 vegans and 142 non-vegetarians. Using linear regression, methylation of CpG sites and genes was categorized or summarized according to various genic/intergenic regions and CpG island-related regions, as well as the promoter. Methylation of genes was measured as the average methylation of available CpG&rsquo s annotated to the nominated region of the respective gene. A permutation method defining the null distribution adapted from Storey et al. was used to adjust for false discovery. Differences in methylation of several CpG sites and genes were detected at a false discovery rate < 0.05 in region-specific and overall analyses. A vegan diet was associated predominantly with hypomethylation of genes, most notably methyltransferase-like 1 (METTL1). Although a limited number of differentially methylated features were detected in the current study, the false discovery method revealed that a much larger proportion of differentially methylated genes and sites exist, and could be detected with a larger sample size. Our findings suggest modest differences in DNA methylation in vegans and non-vegetarians, with a much greater number of detectable significant differences expected with a larger sample. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|