Effect of platelet-rich plasma combined with demineralised bone matrix on bone healing in rabbit ulnar defects
| Autor: | Stylianos Kapetanakis, Vasilios Galanis, Alice Fiska, Thespis Demetriou, Konstantinos J. Kazakos |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Transplantation Heterologous Bone Matrix Ulna Bone healing Bone matrix Bone and Bones 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Bone formation Statistical analysis 030222 orthopedics Bone Transplantation Platelet-Rich Plasma Bone union business.industry dBm 030206 dentistry General Medicine Biomechanical Phenomena Surgery medicine.anatomical_structure Platelet-rich plasma Original Article Rabbits business |
| Zdroj: | Singapore Medical Journal. 58:551-556 |
| ISSN: | 0037-5675 |
| Popis: | Introduction This study evaluates the effect of autologous platelet-rich plasma (PRP) combined with xenogeneic demineralised bone matrix (DBM) on bone healing of critical-size ulnar defects (2-2.5 times the ulnar diameter) in New Zealand White rabbits. Methods Critical-size defects were created unilaterally in the ulna of 36 rabbits, while keeping the contralateral limb intact. They were divided into three groups. In Group A, the defect was filled with autologous PRP and in Group B, with autologous PRP combined with DBM; in Group C, the defect remained empty. The rabbits were euthanised 12 weeks postoperatively. Radiological, biomechanical and histological assessments were carried out and statistical analysis of the results was performed. Results Group B had significantly higher radiological and histological scores than Groups A and C. Defects in Group B showed significant new bone formation, whereas there was minimal or no new bone formation in Groups A and C. Only specimens in Group B showed macroscopic bone union. Biomechanical evaluation of the treated and intact contralateral limbs in Group B showed significant differences. Conclusion In this study, statistically significant enhancement of bone healing was found in critical-size defects treated with PRP and DBM, as shown by radiological findings, gross assessment, and biomechanical and histopathological results. Defects in the two other groups remained unbridged. Therefore, PRP was effective only when it was used in combination with a bone graft. |
| Databáze: | OpenAIRE |
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