Serum antiglycan antibody detection of nonmucinous ovarian cancers by using a printed glycan array

Autor: Viola Heinzelmann-Schwarz, Rosemarie Caduff, Daniel Fink, Darlene R. Goldstein, Marko Vuskovic, Tatiana Pochechueva, Francis Jacob, Margaret E. Huflejt, Marianne Spengler, Nicolai V. Bovin
Přispěvatelé: University of Zurich, Heinzelmann-Schwarz, V
Rok vydání: 2011
Předmět:
Proteomics
epithelial ovarian cancer
Cancer Research
Glycosylation
Expression
chemistry.chemical_compound
0302 clinical medicine
antibodies
Monoclonal-Antibodies
1306 Cancer Research
Prospective Studies
Challenges
Ovarian Neoplasms
0303 health sciences
Middle Aged
Prognosis
Mannan
10174 Clinic for Gynecology
3. Good health
Oncology
Antigen
030220 oncology & carcinogenesis
biomarker
Female
2730 Oncology
Antibody
Glycan
medicine.drug_class
glycan array
Carbohydrate Microarrays
610 Medicine & health
Biology
Monoclonal antibody
Article
Blood-Group
Quantitation
03 medical and health sciences
Polysaccharides
10049 Institute of Pathology and Molecular Pathology
ABO blood group system
Biomarkers
Tumor
Candida-Albicans
medicine
Humans
Neoplasm Staging
030304 developmental biology
Tumor marker
Carcinoma
Transitional Cell
Microarray Analysis
medicine.disease
Molecular biology
Cystadenocarcinoma
Serous
Endometrial Neoplasms
carbohydrates (lipids)
chemistry
Case-Control Studies
Immunoglobulin G
biology.protein
Neoplasm Grading
Ovarian cancer
serum
Biomarkers
Adenocarcinoma
Clear Cell
Follow-Up Studies
Zdroj: International Journal of Cancer
ISSN: 0020-7136
Popis: Epithelial ovarian cancer has the highest mortality rate among gynecological cancers. Altered glycosylation is associated with oncogenic transformation producing tumor-associated carbohydrate antigens. We investigated the potential of natural occurring antiglycan antibodies in the diagnosis of ovarian cancer by using printed glycan array. Antiglycan antibodies bound to 203 chemically synthesized printed glycans were detected via biotin-streptavidin fluorescence system in serum of women with normal operative findings (healthy controls; n = 24) and nonmucinous borderline or ovarian cancer of various FIGO stages (n = 33). Data were validated measuring blood group associated di-, tri and tetrasaccharide antigens on known ABO blood groups. Antiglycan antibodies demonstrated high reproducibility (r(c) > 0.9). Cluster analysis identified repetitive patterns of specific core carbohydrate structures: 11 N-linked glycans, 3 O-linked glycans and 2 glycosphingolipids. Biomarker detection revealed 24 glycans including P-1 (Gal alpha 1-4Gal beta 1-4GlcNAc beta; p < 0.001) significantly discriminating between (low-) malignant tumors and healthy controls. Comparable sensitivity and specificity with tumor marker CA125 was achieved by a panel of multivariate selected and linear combined antiglycan antibody signals (79.2 and 84.8%, respectively). Our findings demonstrate the potential of glycan arrays in the development of a new generation of biomarkers for ovarian cancer.
Databáze: OpenAIRE
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