Molecular cloning and functional expression of a multifunctional triterpene synthase cDNA from a mangrove species Kandelia candel (L.) Druce
| Autor: | Mohammad Basyuni, Takafumi Okabe, Keichiro Oshiro, Shigeyuki Baba, Yutaka Ebizuka, Masaaki Shibuya, Masashi Inafuku, Hirosuke Oku, Hironori Iwasaki |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Gene Expression Plant Science Horticulture Molecular cloning Biochemistry Conserved sequence chemistry.chemical_compound Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence Phylogeny Plant Proteins Lupeol Molecular Structure ATP synthase biology Kandelia candel General Medicine biology.organism_classification Triterpenes chemistry biology.protein Rhizophoraceae Lupeol synthase Lanosterol synthase |
| Zdroj: | Phytochemistry. 67:2517-2524 |
| ISSN: | 0031-9422 |
| Popis: | Homology based PCRs with degenerate primers designed from the conserved sequences among the known oxidosqualene cylases (OSCs) have resulted in cloning of a triterpene synthase (KcMS) from the young roots of Kandelia candel (L.) Druce (Rhizophoraceae). KcMS consists of a 2286 bp open reading frame, which codes for 761 amino acids. The deduced amino acid sequence showed 79% homology to a lupeol synthase from Ricinus communis suggesting it to be a lupeol synthase of K. candel. KcMS was expressed in a lanosterol synthase deficient yeast with the expression vector pYES2 under the control of GAL1 promoter. GC-MS analysis showed that the transformant accumulated a mixture of lupeol, beta-amyrin and alpha-amyrin in a 2:1:1 ratio, indicating that KcMS encodes a multifunctional triterpene synthase, although it showed high sequence homology to a R. communis lupeol synthase. This is the first OSC cloning from mangrove tree species. |
| Databáze: | OpenAIRE |
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