Endogenous Endomorphin-2 Contributes to Spinal ĸ-Opioid Antinociception
| Autor: | Yugo Tagaito, Satoshi Toyama, Megumi Shimoyama, Naohito Shimoyama |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Agonist medicine.drug_class Enkephalin Methionine Receptors Opioid mu Pain Pharmacology Dynorphins Rats Sprague-Dawley chemistry.chemical_compound Opioid receptor medicine Animals Opioid peptide Receptor Endogenous opioid business.industry Receptors Opioid kappa 3 4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide (trans)-Isomer Dynorphin A General Medicine Analgesics Non-Narcotic Rats Nociception Spinal Cord Opioid chemistry Anesthesia business Oligopeptides medicine.drug |
| Zdroj: | Pharmacology. 89:145-148 |
| ISSN: | 1423-0313 0031-7012 |
| DOI: | 10.1159/000336770 |
| Popis: | Background/Aims: Multiple opioid receptor (OR) types and endogenous opioid peptides exist in the spinal dorsal horn and there may be interactions among these receptor types that involve opioid peptides. In a previous study we observed that antinociceptive effects of the selective ĸ-opioid receptor (ĸOR) agonist, U50,488H, was attenuated in µ-opioid receptor (µOR) knockout mice as compared to wild-type mice when administered spinally. This suggests that an interaction between ĸORs and µORs exits in the spinal cord. The present study was aimed at investigating whether endogenous opioid peptides were involved in such interaction. Methods: We examined whether the presence of antibodies to endogenous opioid peptides, endomorphin-2, met-enkephalin and dynorphin A affected the antinociceptive effects of spinal U50,488H in rats. The tail-flick test was used to assess pain thresholds. Results: The increase in tail-flick latency after spinal U50,488H was attenuated when the rats were pretreated intrathecally with antiserum against endomorphin-2. Pretreatments with antisera against met-enkephalin and dynorphin A had no effect on U50,488H antinociception. The antisera alone did not affect pain threshold. Conclusion: The results suggest that endomorphin-2, an endogenous opioid peptide highly selective to the µOR, plays a role in antinociception induced by ĸOR activation in the spinal cord. |
| Databáze: | OpenAIRE |
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