Cryopreservation studies of Campylobacter
| Autor: | Charles K. Mills, Robert L. Gherna |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Time Factors
food.ingredient medicine.disease_cause Campylobacter jejuni General Biochemistry Genetics and Molecular Biology Cryopreservation Microbiology chemistry.chemical_compound Freeze-drying Campylobacter fetus food Species Specificity Skimmed milk Congelation Glycerol medicine Food science biology Campylobacter General Medicine biology.organism_classification Culture Media Freeze Drying chemistry General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 25:148-152 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/0011-2240(88)90008-9 |
| Popis: | Seven strains of Campylobacter fetus ss. fetus, one of Campylobacter fetus ss. venerealis, and one of Campylobacter jejuni were preserved using a variety of cryopreservation methods. Organisms were frozen to -150 degrees C in a liquid nitrogen refrigerator, in the freezer compartment of a refrigerator (-20 degrees C), and in a mechanical freezer (-65 degrees C). In the latter two cases, viabilities of the organisms were compared after being frozen in Brucella Albimi broth and 10% glycerol. Viabilities were also examined after Campylobacter species were freeze-dried using rapid or slow cooling, using sucrose or skim milk as cryoprotective agents and in bulb-type vials on a manifold or batch vials. Preservation in liquid nitrogen resulted in no loss in viability after 4 years storage. When Campylobacter species were frozen at -20 degrees C, no cells were recovered after 1 month storage in Brucella Albimi broth or seven months in glycerol. A 6.5 log decrease in viability resulted after organisms were frozen at -65 degrees and subsequently stored at the same temperature for 2 years. In this case, glycerol had no protective advantage over Brucella Albimi broth. Postpreservation viability of organisms cooled slowly was two logs higher than those cooled rapidly prior to freeze-drying. When skim milk or sucrose were employed as cryoprotective agents during freeze-drying, equal viabilities resulted. Equivalent viabilities were also demonstrated when the bulb type or "batch" vials were utilized for freeze-drying. No significant differences were observed between the viabilities of the three species when a given cryopreservation method was employed. |
| Databáze: | OpenAIRE |
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