Identification of novel human papillomavirus lineages and sublineages in HIV/HPV-coinfected pregnant women by next-generation sequencing
| Autor: | Esmeralda A. Soares, Juliana D. Siqueira, Brunna M. Alves, Carolina Furtado, Marcelo A. Soares, Elizabeth S. Machado, Isabel M. Prellwitz, Ângela R. Meyrelles, Héctor N. Seuánez |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine HIV Infections Genome Viral Biology Genome DNA sequencing Cohort Studies 03 medical and health sciences Acquired immunodeficiency syndrome (AIDS) Pregnancy Virology medicine Humans Pregnancy Complications Infectious Papillomaviridae Cervical cancer Phylogenetic tree Coinfection Papillomavirus Infections High-Throughput Nucleotide Sequencing virus diseases medicine.disease biology.organism_classification 030104 developmental biology Cohort Female |
| Zdroj: | Virology. 493:202-208 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2016.03.027 |
| Popis: | Infection by human papillomavirus (HPV) is a necessary condition for development of cervical cancer, and has also been associated with malignancies of other body anatomical sites. Specific HPV types have been associated with premalignant lesions and invasive carcinoma, but mounting evidence suggests that within-type lineages and sublineages also display distinct biological characteristics associated with persistent infections and evolution to cervical cancer. In the present study, we have assessed HPV multiple infection and variation from a cohort of highly susceptible, HIV(+) pregnant women using next-generation sequencing and an in-house pipeline for HPV full-length genome assembly. Seventy-two consensus sequences representing complete or near-complete (>97%) HPV genomes were assembled, spanning 28 different types. Genetic distance and phylogenetic analyses allowed us to propose the classification of novel HPV lineages and sublineages across nine HPV types, including two high-risk types. HPV diversity may be a hallmark of immunosuppressed patients upon HIV infection and AIDS progression. |
| Databáze: | OpenAIRE |
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