The Cytoplasmic Domain of Xenopus NF-Protocadherin Interacts with TAF1/Set
| Autor: | Mike A Heggem, Roger Bradley |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Embryo
Nonmammalian animal structures Morpholino Chromosomal Proteins Non-Histone Recombinant Fusion Proteins Molecular Sequence Data Xenopus Protocadherin Apoptosis Xenopus Proteins Biology General Biochemistry Genetics and Molecular Biology Xenopus laevis 03 medical and health sciences Cytosol 0302 clinical medicine Ectoderm Animals Humans Histone Chaperones Amino Acid Sequence RNA Messenger Molecular Biology 030304 developmental biology 0303 health sciences Cadherin Cell adhesion molecule Gene Expression Regulation Developmental Cell Differentiation Embryo Cell Biology Cadherins biology.organism_classification Immunohistochemistry Molecular biology Protocadherins Protein Structure Tertiary 3. Good health Cell biology DNA-Binding Proteins TAF1 Antisense Elements (Genetics) Cytoplasm Mutation embryonic structures 030217 neurology & neurosurgery HeLa Cells Transcription Factors Developmental Biology |
| Zdroj: | Developmental Cell. 4(3):419-429 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/s1534-5807(03)00036-4 |
| Popis: | Protocadherins are members of the cadherin superfamily of cell adhesion molecules proposed to play important roles in early development, but whose mechanisms of action are largely unknown. We examined the function of NF-protocadherin (NFPC), a novel cell adhesion molecule essential for the histogenesis of the embryonic ectoderm in Xenopus, and demonstrate that the cellular protein TAF1, previously identified as a histone-associated protein, binds the NFPC cytoplasmic domain. NFPC and TAF1 coprecipitate from embryo extracts when ectopically expressed, and TAF1 can rescue the ectodermal disruptions caused by a dominant-negative NFPC construct lacking the extracellular domain. Furthermore, disruptions in either NFPC or TAF1 expression, using NFPC- or TAF1-specific antisense morpholinos, result in essentially identical ectodermal defects. These results indicate a role for TAF1 in the differentiation of the embryonic ectoderm, as a cytosolic cofactor of NFPC. |
| Databáze: | OpenAIRE |
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