Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera
| Autor: | Hao-Kang Jia, Yu-Di Liu, Mao-Lin Hou, Yu-Tong Miao, Yao Deng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0301 basic medicine Saliva Proteome Physiology Hydrolases Gene Expression lcsh:Medicine Biochemistry Salivary Glands Tandem Mass Spectrometry Gene expression Medicine and Health Sciences Calcium ion binding lcsh:Science Multidisciplinary Salivary gland Effector Body Fluids Enzymes medicine.anatomical_structure Insect Proteins Anatomy Research Article Cell Binding Cell Physiology Motor Proteins Protein domain Biology DNA-binding protein Hemiptera 03 medical and health sciences Exocrine Glands Protein Domains Molecular Motors DNA-binding proteins Genetics medicine Animals Gene Regulation Biology and life sciences lcsh:R Proteins Cell Biology Regulatory Proteins 030104 developmental biology Secretory protein Enzymology lcsh:Q Digestive System Transcription Factors |
| Zdroj: | PLoS ONE, Vol 13, Iss 5, p e0193831 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 proteins were identified, which were divided into 8 function categories, including enzymes, transporter, calcium ion binding protein, salivary sheath protein, cytoskeleton protein, DNA-, RNA-, and protein-binding or regulating proteins, other non-enzyme proteins and unknown proteins. Gene expression pattern of 11 secretory proteins were analyzed by real time quantitative-PCR. We detected the mucin-like protein, which had a unique expression level in salivary gland, most likely to be a candidate effector involved in regulation of plant defense. This study identified the watery saliva component of S. furcifera and it provided a list of proteins which may play a role in interaction between S. furcifera and rice. |
| Databáze: | OpenAIRE |
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