Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice

Autor:	Na Zhao, Weiwei Liu, Jia Wang, Wenwen Zhu, Beixing Liu, Rui Zheng, Dalu Wang
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Microbiology (medical) Immunology Pneumonia Viral Macrophage polarization Administration Oral Inflammation rsv Respiratory Syncytial Virus Infections Infectious and parasitic diseases RC109-216 Microbiology immune response Interferon-gamma Mice Immune system Lactobacillus medicine Animals Clostridium butyricum Mice Inbred BALB C Lung gastrointestinal microbiota biology Interleukin-17 biology.organism_classification medicine.disease butyrate Gastrointestinal Microbiome macrophages Infectious Diseases medicine.anatomical_structure Respiratory Syncytial Virus Human clostridium butyricum Streptomycin Dysbiosis Parasitology medicine.symptom Interleukin-5 Respiratory tract Research Article Research Paper
Zdroj:	Virulence, Vol 12, Iss 1, Pp 2133-2148 (2021) Virulence article-version (VoR) Version of Record
ISSN:	2150-5608 2150-5594
Popis:	Changes in the intestinal microbiota indirectly impact the health of mucosa distal to the intestine, particularly the respiratory tract. However, the effects of intestinal microbiota dysbiosis on the regulation of respiratory syncytial virus (RSV) infection are not clear. In this study, we examined the effects of altering the intestinal microbiota on the pulmonary immune response against RSV infection. BALB/c mice were treated with streptomycin before infection with RSV to study the altered immune response. The ingestion of streptomycin led to a marked alteration in the intestinal microbiota with a reduced abundance of Lactobacillus and Clostridium genera, followed by greatly aggravated pulmonary inflammation in response to RSV infection. This aggravated inflammation was associated with a dysregulated immune response against RSV infection, characterized by the increased expression of IFN-γ and IL-17 and increased pulmonary M1-like macrophage polarization, and decreased expression of IL-5. Supplementation of Clostridium butyricum (CB) prevented aggravated inflammation and the dysregulated immune response characterized by greater M2 polarization of pulmonary macrophages and decreased release of IFN-γ and IL-17 as well as increased IL-5 levels. Furthermore, in vitro and in vivo experiments identified that butyrate, the main metabolite produced by CB, promoted M2 polarization of macrophages in RSV-infected mice exposed to streptomycin. Together, these results demonstrate the mechanism by which intestinal microbiota modulate the pulmonary immune response to RSV infection.
Databáze:	OpenAIRE
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