Thyroxine increases Serca2 and Ryr2 gene expression in heart failure rats with euthyroid sick syndrome
| Autor: | Regiane Marques Castro Olimpio, Gláucia Maria Ferreira da Silva Mazeto, Maria Teresa De Sibio, Antonio Carlos Cicogna, Miriane de Oliveira, Renata de Azevedo Melo Luvizotto, Dijon Henrique Salomé de Campos, Denise Perone, Célia Regina Nogueira, Carlos Roberto Padovani, Fábio V. G. Campanha, Fernanda Cristina Fontes Moretto |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Mato Grosso (UFMT) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Gene Expression heart failure lcsh:Medicine Heart failure 030204 cardiovascular system & hematology Ryanodine receptor 2 lcsh:Diseases of the endocrine glands. Clinical endocrinology Sarcoplasmic Reticulum Calcium-Transporting ATPases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine.artery calcium channels triiodothyronine medicine Animals Thoracic aorta RNA Messenger Thoracotomy Rats Wistar Triiodothyronine lcsh:RC648-665 business.industry lcsh:R Therapeutic use Ryanodine Receptor Calcium Release Channel Reverse triiodothyronine Aortic Valve Stenosis medicine.disease Euthyroid Sick Syndromes Thyroxine Calcium channels 030104 developmental biology Endocrinology chemistry Aortic valve stenosis therapeutic use Models Animal business Euthyroid sick syndrome |
| Zdroj: | Archives of Endocrinology and Metabolism, Vol 60, Iss 6, Pp 582-586 (2016) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Archives of Endocrinology and Metabolism v.60 n.6 2016 Arquivos de Endocrinologia e Metabolismo Sociedade Brasileira de Endocrinologia e Metabologia (SBEM) instacron:SBEM Archives of Endocrinology and Metabolism, Volume: 60, Issue: 6, Pages: 582-586, Published: 10 OCT 2016 |
| ISSN: | 2359-4292 2359-3997 |
| Popis: | Made available in DSpace on 2018-12-11T17:08:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2019-10-09T18:30:42Z : No. of bitstreams: 1 S2359-39972016000600582.pdf: 208346 bytes, checksum: d4024e8a967f9479c5370d43b346a841 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: The current study was aimed at analyzing sarcoplasmic reticulum Ca2+ ATPase (Serca2) and ryanodine receptor type 2 (Ryr2) gene expression in rats subjected to surgery that induced HF and were subsequently treated with T4 using physiological doses. Materials and methods: HF was induced in 18 male Wistar rats by clipping the ascending thoracic aorta to generate aortic stenosis (HFS group), while the control group (9-sham) underwent thoracotomy. After 21 weeks, the HFS group was subdivided into two subgroups. One group (9 Wistar rats) with HF received 1.0 µg of T4/100 g of body weight for five consecutive days (HFS/T4); the other group (9 Wistar rats) received isotonic saline solution (HFS/S). The animals were sacrificed after this treatment and examined for signs of HF. Samples from the left ventricles of these animals were analyzed by RT-qPCR for the expression of Serca2 and Ryr2 genes. Results: Rats with HF developed euthyroid sick syndrome (ESS) and treatment with T4 restored the T3 values to the Sham level and increased Serca2 and Ryr2 gene expression, thereby demonstrating a possible benefit of T4 treatment for heart function in ESS associated with HF. Conclusion: The T4 treatment can potentially normalize the levels of T3 as well elevated Serca2 and Ryr2 gene expression in the myocardium in heart failure rats with euthyroid sick syndrome. Departamento de Clínica Médica Unidade de Pesquisa Experimental (Unipex) Faculdade de Medicina de Botucatu Universidade Estadual Paulista (Unesp) Instituto de Ciências da Saúde Universidade Federal de Mato Grosso (UFMT) Departamento de Bioestatística Instituto de Biociências Universidade Estadual Paulista (Unesp) Departamento de Clínica Médica Unidade de Pesquisa Experimental (Unipex) Faculdade de Medicina de Botucatu Universidade Estadual Paulista (Unesp) Departamento de Bioestatística Instituto de Biociências Universidade Estadual Paulista (Unesp) FAPESP: 2007/55902-8 |
| Databáze: | OpenAIRE |
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