Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
| Autor: | Denis Zakharkin, Victor V. Revin, Nelli Atykyan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Sonication lcsh:Biotechnology Bioethanol 02 engineering and technology Cellulase Raw material 01 natural sciences Applied Microbiology and Biotechnology Hydrolysis chemistry.chemical_compound Ligsnocelluloses 010608 biotechnology Enzymatic hydrolysis lcsh:TP248.13-248.65 Yeast Saccharomyces cerevisiae lcsh:QH301-705.5 Chromatography Ethanol biology Chemistry 021001 nanoscience & nanotechnology Biochemistry lcsh:Biology (General) visual_art visual_art.visual_art_medium biology.protein Fermentation Sawdust 0210 nano-technology Biotechnology |
| Zdroj: | Electronic Journal of Biotechnology, Vol 20, Iss C, Pp 14-19 (2016) Electronic Journal of Biotechnology v.19 n.2 2016 SciELO Chile CONICYT Chile instacron:CONICYT Electronic Journal of Biotechnology, Volume: 19, Issue: 2, Pages: 14-19, Published: MAR 2016 |
| ISSN: | 0717-3458 |
| Popis: | Background: A study of the correlation between the particle size of lignocellulosic substrates and ultrasound pretreatment on the efficiency of further enzymatic hydrolysis and fermentation to ethanol. Results: The maximum concentrations of glucose and, to a lesser extent, di- and trisaccharides were obtained in a series of experiments with 48-h enzymatic hydrolysis of pine raw materials ground at 380 - 400 rpm for 30 min. The highest glucose yield was observed at the end of the hydrolysis with a cellulase dosage of 10 mg of protein (204 ± 21 units CMCase per g of sawdust). The greatest enzymatic hydrolysis efficiency was observed in a sample that combined two-stage grinding at 400 rpm with ultrasonic treatment for 5 - 10 min at a power of 10 W per kg of sawdust. The glucose yield in this case (35.5 g glucose l -1 ) increased twofold compared to ground substrate without further preparation. Conclusions: using a mechanical two-stage grinding of lignocellulosic raw materials with ultrasonication increases the efficiency of subsequent enzymatic hydrolysis and fermentation. |
| Databáze: | OpenAIRE |
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