Whole tissue cervical mapping of HPV infection: Molecular evidence for focal latent HPV infection in humans
| Autor: | Hans Svanholm, John Doorbar, Torben Steiniche, Wim Gv Quint, Heather Griffin, Anne Hammer, Maurits N. C. de Koning, Patti E. Gravitt, Jan Blaakær, Else Mejlgaard |
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| Přispěvatelé: | Doorbar, John [0000-0002-4027-102X], Griffin, Heather [0000-0002-4601-3064], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Oncology
medicine.medical_specialty HPV CERVICAL ABNORMALITY Molecular biology medicine.medical_treatment Molecular evidence Cervix Uteri Stain Article lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Virology Internal medicine HE (hematoxylin and eosin) Virus latency DNA (deoxyribonucleic acid) medicine Humans lcsh:RC109-216 030212 general & internal medicine Latency (engineering) Cervix Papillomaviridae Aged mRNA (messenger ribonucleic acid) Uterine cervical neoplasm HIV (human immunodeficiency virus) Hysterectomy business.industry Papillomavirus Infections HPV infection Papillomavirus Middle Aged medicine.disease Infectious Diseases medicine.anatomical_structure 030220 oncology & carcinogenesis Carrier State HPV (human papilloma virus) Female business |
| Zdroj: | Papillomavirus Research, Vol 7, Iss, Pp 82-87 (2019) Hammer, A, de Koning, M NC, Blaakaer, J, Steiniche, T, Doorbar, J, Griffin, H, Mejlgaard, E, Svanholm, H, Quint, W GV & Gravitt, P E 2019, ' Whole tissue cervical mapping of HPV infection : Molecular evidence for focal latent HPV infection in humans ', Papillomavirus Research, vol. 7, no. June, pp. 82-87 . https://doi.org/10.1016/j.pvr.2019.02.004 Hammer, A, de Koning, M NC, Blaakaer, J, Steiniche, T, Doorbar, J, Griffin, H, Mejlgaard, E, Svanholm, H, Quint, W GV & Gravitt, P E 2019, ' Whole tissue cervical mapping of HPV infection : Molecular evidence for focal latent HPV infection in humans ', Papillomavirus Research, vol. 7, pp. 82-87 . https://doi.org/10.1016/j.pvr.2019.02.004 Hammer, A, de Koning, M, Doorbar, J, Griffin, H, Blaakær, J, Steiniche, T, Mejlgaard, E, Svanholm, H, Quint, W & Gravitt, P 2019, ' Whole tissue cervical mapping of HPV infection: molecular evidence for focal latent HPV infection in human ', Papillomavirus research (Amsterdam, Netherlands) . Papillomavirus Research |
| ISSN: | 2405-8521 |
| Popis: | In this study, we aimed to provide molecular evidence of HPV latency in humans and discuss potential challenges of conducting studies on latency. We analyzed the entire cervix of two women who underwent hysterectomy unrelated to cervical abnormality. The cervices were sectioned into 242 and 186 sets respectively, and each set was tested separately for HPV using the SPF10-PCR-DEIA-LiPA25 system. To identify whether there was any evidence of transforming or productive infection, we used the biomarkers E4 and P16INK4a to stain slides immediately adjacent to HPV-positive sections. HPV was detected in both cervices. In patient 1, 1/242 sets was positive for HPV31. In patient 2, 13/186 sets were positive for HPV18 and 1/186 was positive for HPV53. The infection was very focal in both patients, and there was no sign of a transforming or productive infection, as evaluated by the markers E4 and P16INK4a. Had we only analyzed one set from each block, the probability of detecting the infection would have been 32.3% and 2%, respectively.Our findings support the idea that HPV may be able to establish latency in the human cervix; however, the risk associated with a latent HPV infection remains unclear. Keywords: HPV, Papillomavirus, Humans, Virus latency, Uterine cervical neoplasm, Molecular biology |
| Databáze: | OpenAIRE |
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