Identity of Rat Kidney and Urine Alkaline Phosphatase Actions and Relation to the Elfect of Nitrofuranfuradroxyl
| Autor: | Abdel Hamid Nf, El Din Awad Om |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Nitrofurans
Phosphatase chemistry.chemical_element Urine Zinc Kidney Biochemistry medicine Animals Magnesium Semicarbazones chemistry.chemical_classification Alkaline Phosphatase In vitro Rats Kinetics Enzyme medicine.anatomical_structure Liver chemistry Organ Specificity Glycerophosphates Alkaline phosphatase |
| Zdroj: | Enzyme. 20:221-233 |
| ISSN: | 2504-2564 0013-9432 |
| DOI: | 10.1159/000458943 |
| Popis: | The activities of alkaline phosphatases of kidney and urine appear similar and identical in action. Both are magnesium-dependent and inhibited by higher concentrations of 2-glycerophosphate. A band of similar mobility is obtained on electrophoresis of kidney and urine enzymes. In vitro magnesium competitively inhibits their activities when the ratio of Mg-+2 ions/2-glycerophosphate is above a definite level. Their activities are not affected by exogenous zinc and follow the Michaelis-Menten equation only when attention is given to the ratio of Mg-+2 ions/substrate. Nitrofuranfuradroxyl is a mixed type inhibitor showing a second-order rate of reaction with kidney and urine phosphatases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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