Hymenobacter oligotrophus sp. nov., isolated from a contaminated agar plate
| Autor: | Jia Liu, Kun Qin, Ziyan Wei, Fang Peng, Yao Huang, Yingchao Geng, Yumin Zhang, Jin Tian |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine food.ingredient Sodium Chloride medicine.disease_cause DNA Ribosomal 010603 evolutionary biology 01 natural sciences Microbiology Agar plate 03 medical and health sciences Cytosol food RNA Ribosomal 16S Hymenobacter Optimum growth Environmental Microbiology medicine Cluster Analysis Molecular Biology Phospholipids Phylogeny Base Composition biology Bacteroidetes Chemistry Fatty Acids Quinones Temperature Hymenobacter coalescens Nucleic Acid Hybridization Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Culture Media Oligotrophus Hymenobacter koreensis Agar 030104 developmental biology Locomotion Bacteria |
| Zdroj: | Antonie van Leeuwenhoek. 112:1533-1544 |
| ISSN: | 1572-9699 0003-6072 |
| Popis: | A taxonomic study of a Gram-stain negative, rod-shaped, motile, asporogenous, catalase- and oxidase-positive bacterium, sh-6T, forming pink-red colonies, isolated from a contaminated R2A plate in the laboratory was performed. Its optimum growth temperature was determined to be 28 °C in the absence of NaCl on R2A plates. On the basis of 16S rRNA gene sequence analysis, strain sh-6T belongs to the genus Hymenobacter and is closely related to Hymenobacter deserti ZLB-3T (95.05%), Hymenobacter paludis KBP-30T (94.96%), Hymenobacter coalescens WW84T (94.04%), Hymenobacter gummosus ANT-18T (93.38%), Hymenobacter ocellatus Myx2105T (93.70%), Hymenobacter jeollabukensis 1-3-3-8T (93.48%) and Hymenobacter koreensis GYR3077T (93.21%). Comparison of the genome of strain sh-6T and that of H. gummosus ANT-18T gave digital DNA–DNA hybridization and Average Nucleotide Identity values of 20.6% and 78.4%, respectively. The respiratory isoprenoid quinone and polyamine component were identified as MK-7 and sym-homospermidine, respectively. The major cellular fatty acids identified as iso-C15:0, summed feature 4 (iso-C17:1 I/anteiso B), iso-C16:0, iso-C17:0 3-OH and iso-C17:0. The major polar lipid of strain sh-6T determined to be phosphatidylethanolamine. The DNA G+C content was determined to be 60.5 mol%. On the basis of the evidence presented in this study, a novel species of the genus Hymenobacter, Hymenobacter oligotrophus sp. nov., is proposed, with the type strain sh-6T (= CCTCC AB 2016064T = KCTC 62345T). |
| Databáze: | OpenAIRE |
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