Physical and genetic map of the Lactococcus lactis subsp. cremoris MG1363 chromosome: comparison with that of Lactococcus lactis subsp. lactis IL 1403 reveals a large genome inversion
| Autor: | Paul Ritzenthaler, Martine Lautier, P Le Bourgeois, M J Gasson, L van den Berghe |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Genetic Markers Restriction Mapping DNA Ribosomal Microbiology SmaI Restriction fragment Plasmid Species Specificity Operon Bacteriophages Deoxyribonucleases Type II Site-Specific Molecular Biology Genetics biology Lactococcus lactis subsp cremoris Lactococcus lactis Chromosome Mapping Nucleic Acid Hybridization food and beverages Chromosome Chromosomes Bacterial biology.organism_classification Molecular biology Mutagenesis Insertional Restriction site biology.protein RRNA Operon Research Article |
| Zdroj: | Journal of Bacteriology. 177:2840-2850 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.177.10.2840-2850.1995 |
| Popis: | A physical and genetic map of the chromosome of the Lactococcus lactis subsp. cremoris reference strain MG1363 was established. The physical map was constructed for NotI, ApaI, and SmaI enzymes by using a strategy that combines creation of new rare restriction sites by the random-integration vector pRL1 and ordering of restriction fragments by indirect end-labeling experiments. The MG1363 chromosome appeared to be circular and 2,560 kb long. Seventy-seven chromosomal markers were located on the physical map by hybridization experiments. Integration via homologous recombination of pRC1-derived plasmids allowed a more precise location of some lactococcal genes and determination of their orientation on the chromosome. The MG1363 chromosome contains six rRNA operons; five are clustered within 15% of the chromosome and transcribed in the same direction. Comparison of the L. lactis subsp. cremoris MG1363 physical map with those of the two L. lactis subsp. lactis strains IL1403 and DL11 revealed a high degree of restriction polymorphism. At the genetic organization level, despite an overall conservation of gene organization, strain MG1363 presents a large inversion of half of the genome in the region containing the rRNA operons. |
| Databáze: | OpenAIRE |
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