Idiopathic neutropenia of childhood is associated with Fas/FasL expression☆
| Autor: | Harvey J. Cohen, Michael Jeng, Kari C. Nadeau, Jae-Won Joh, Wendy Wong, Angel Callejas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Fas Ligand Protein
Neutropenia Neutrophils Neutrophile medicine.medical_treatment Immunology Apoptosis HL-60 Cells Granulocyte Fas ligand Article hemic and lymphatic diseases medicine Immunology and Allergy Humans fas Receptor Child Leukopenia business.industry Reverse Transcriptase Polymerase Chain Reaction Infant medicine.disease Flow Cytometry medicine.anatomical_structure Cytokine Child Preschool Immunoglobulin G Cytokines Tumor necrosis factor alpha medicine.symptom business |
| Popis: | Idiopathic neutropenia (IN) in children is characterized by decreased neutrophil counts (1500/microl), can be acute or chronic (greater than 6 months duration). The pathophysiology is not well understood; therefore, potential mechanisms of pediatric IN were investigated. An increase in Fas transcripts in neutrophils of IN patients compared to age-matched healthy control (HC) neutrophils was observed (p0.005). Increased expression of Fas protein was found in IN neutrophils, while Fas surface expression on other immune cells was similar. Plasma from acute IN patients had higher protein levels of soluble FasL than chronic IN patients. When HC neutrophils were incubated in plasma from IN patients, greater rates of apoptosis were observed. Biochemical studies suggest the apoptotic factor(s) in plasma is heat-sensitive, non-IgG, and 12-50 kD protein. Addition of anti-sFasL blocking antibodies to patient plasma caused a statistically significant decrease in neutrophil apoptosis. These studies show that the Fas/FasL pathway could be associated with neutrophil apoptosis in childhood IN. |
| Databáze: | OpenAIRE |
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