Diminished expression of complement regulatory proteins (CD55 and CD59) in lymphocytes from systemic lupus erythematosus patients with lymphopenia
| Autor: | J. Jakez-Ocampo, A Ruiz-Argüelles, Yvonne Richaud-Patin, Javier Cabiedes, Elena Soto-Vega, E Carrillo-Maravilla, D Elías-López, I García-Valladares, Y Atisha-Fregoso, L Llorente |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty CD3 Complex T-Lymphocytes CD3 Antigens CD19 Down-Regulation CD59 Antigens Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena CD59 030204 cardiovascular system & hematology CD19 03 medical and health sciences 0302 clinical medicine Rheumatology Antigen immune system diseases Lymphopenia Internal medicine medicine Humans Lupus Erythematosus Systemic Serologic Tests skin and connective tissue diseases 030203 arthritis & rheumatology B-Lymphocytes Lupus erythematosus CD55 Antigens biology business.industry Autoantibody Middle Aged Flow Cytometry medicine.disease Endocrinology Antibodies Antinuclear Case-Control Studies Immunology biology.protein Female Antibody business Anti-SSA/Ro autoantibodies |
| Zdroj: | Lupus. 15:600-605 |
| ISSN: | 1477-0962 0961-2033 |
| DOI: | 10.1177/0961203306071916 |
| Popis: | CD55 and CD59 are glycophosphatidylinositol-anchored proteins with complement inhibitory properties. Lymphopenia in systemic lupus erythematosus (SLE) has been associated with autoantibodies targeting nuclear antigens. The aim of this study was to evaluate the surface density of CD55 and CD59 in T and B lymphocytes from patients with SLE and lymphopenia and its possible correlation with the presence of common SLE autoantibodies. Flow cytometric analyses were performed on CD55 and CD59 stained CD3 and CD19 cells from 40 SLE patients, 30 with lymphopenia and 10 without it, and 25 healthy controls. Autoantibodies were detected in the sera by enzyme linked immunosorbent assay. The mean fluorescence intensity of CD55 and CD59 in T and B cells was significantly diminished in SLE patients with lymphopenia when compared with healthy subjects. Interestingly, the opposite was found in T and B cells from non-lymphopenic SLE patients. Although there was no correlation between CD55 and CD59 surface density and the presence of any specificity of the autoantibodies tested, higher titres of anti-dsDNA, anti-SM and anti-ribosomal p antibodies were significantly associated with lymphopenia. The deficiency of CD55 and CD59 expression may play a role in the pathophysiology of lymphopenia, most likely by increasing the susceptibility of cells to complement mediated cytolysis. |
| Databáze: | OpenAIRE |
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