Distinct tRNA Accommodation Intermediates Observed on the Ribosome with the Antibiotics Hygromycin A and A201A
| Autor: | Yury S. Polikanov, Thomas A. Steitz, Benjamin J. Burnett, Scott C. Blanchard, Agata L. Starosta, George P. Dinos, Wanli Lu, Daniel S. Terry, Daniel N. Wilson, Roger B. Altman, Manuel F. Juette, Kevin A. Reynolds |
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| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Peptidyl transferase Protein Conformation Ribosome Subunits Small Bacterial Ribosome Subunits Large Bacterial Crystallography X-Ray Ribosome Article chemistry.chemical_compound Protein structure RNA Transfer Disk Diffusion Antimicrobial Tests Drug Resistance Bacterial Escherichia coli Molecular Biology biology Thermus thermophilus Hydrogen Bonding Cell Biology biology.organism_classification Anti-Bacterial Agents 3. Good health carbohydrates (lipids) A-site Aminoglycosides chemistry Biochemistry Cinnamates Ribosome Subunits Transfer RNA biology.protein Hygromycin B |
| Zdroj: | Molecular Cell. 58:832-844 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2015.04.014 |
| Popis: | The increase in multi-drug resistant bacteria is limiting the effectiveness of currently approved antibiotics, leading to a renewed interest in antibiotics with distinct chemical scaffolds. We have solved the structures of the Thermus thermophilus 70S ribosome with A-, P- and E-site tRNAs bound, and in complex with either the aminocyclitol-containing antibiotic hygromycin A (HygA) or the nucleoside antibiotic A201A. Both antibiotics bind at the peptidyl transferase center and sterically occlude the CCA-end of the A-tRNA from entering the A-site of the peptidyl transferase center. Single-molecule Förster resonance energy transfer (smFRET) experiments reveal that HygA and A201A specifically interfere with full accommodation of the A-tRNA, leading to the presence of tRNA accommodation intermediates, and thereby inhibiting peptide bond formation. Thus, our results provide not only insight into the mechanism of action of HygA and A201A, but also into the fundamental process of tRNA accommodation during protein synthesis. |
| Databáze: | OpenAIRE |
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