Antileukemic Cell Proliferation of Active Compounds from Kaffir Lime (Citrus hystrix) Leaves
| Autor: | Fah Chueahongthong, Songyot Anuchapreeda, Riki Anzawa, Pawaret Panyajai, Siriporn Okonogi, Methee Rungrojsakul, Singkome Tima, Natsima Viriyaadhammaa, Aroonchai Saiai, Toyonobu Usuki, Chadarat Ampasavate |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell cycle checkpoint
food.ingredient Wilms’ tumor 1 antiproliferation Pharmaceutical Science 01 natural sciences Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound Phytol phytol food lcsh:Organic chemistry Drug Discovery Physical and Theoretical Chemistry 030304 developmental biology Lupeol 0303 health sciences lupeol Chemistry Cell growth kaffir lime Organic Chemistry leukemia food and beverages Cell cycle Kaffir Lime 0104 chemical sciences 010404 medicinal & biomolecular chemistry Biochemistry Chemistry (miscellaneous) Cell culture Molecular Medicine Trypan blue |
| Zdroj: | Molecules Volume 25 Issue 6 Molecules, Vol 25, Iss 6, p 1300 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25061300 |
| Popis: | Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated via inhibition of Wilms&rsquo tumor 1 (WT1), which is a protein that involves in leukemic cell proliferation. In addition, the compounds were investigated for their effects on WT1 gene expression using real time RT-PCR and Western blotting. Cell cycle arrest and total cell number were investigated using flow cytometry and trypan blue exclusion method, respectively. The results demonstrated that the hexane fractionated extract had the greatest inhibitory effect on WT1 gene expression of many leukemic cell lines and significantly decreased WT1 protein levels of K562 cells (representative of the leukemic cells), in a dose- and time-dependent manner. Subfraction No. 9 (F9) after partial purification of hexane fractioned extract showed the highest suppression on WT1 protein and suppressed cell cycle at G2/M. The organic compounds were isolated from F9 and identified as phytol and lupeol. The bioassays confirmed antiproliferative activities of natural products phytol and lupeol. The results demonstrated anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation. |
| Databáze: | OpenAIRE |
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