Use of a molecular diagnostic test in AFB smear positive tuberculosis suspects greatly reduces time to detection of multidrug resistant tuberculosis
| Autor: | Michael K. Leonard, Rusudan Apsindzelashvili, Henry M. Blumberg, Russell R. Kempker, Iagor Kalandadze, Ekaterina Kurbatova, Nino Bablishvili, Nestani Tukvadze, Maia Kipiani |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Bacterial Diseases
Antitubercular Agents lcsh:Medicine Drug resistance Global Health Gastroenterology 0302 clinical medicine Tuberculosis Multidrug-Resistant 030212 general & internal medicine lcsh:Science 0303 health sciences Multidisciplinary medicine.diagnostic_test biology Multi-drug-resistant tuberculosis Isoniazid 3. Good health Infectious Diseases Molecular Diagnostic Techniques Medicine medicine.symptom medicine.drug Research Article medicine.medical_specialty Tuberculosis Georgia Microbiology Sputum culture Mycobacterium tuberculosis 03 medical and health sciences Diagnostic Medicine Internal medicine medicine Humans Biology 030306 microbiology business.industry lcsh:R Sputum Extensively drug-resistant tuberculosis Reproducibility of Results Tropical Diseases (Non-Neglected) medicine.disease biology.organism_classification Immunology Mutation lcsh:Q business |
| Zdroj: | PLoS ONE, Vol 7, Iss 2, p e31563 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background The WHO has recommended the implementation of rapid diagnostic tests to detect and help combat M/XDR tuberculosis (TB). There are limited data on the performance and impact of these tests in field settings. Methods The performance of the commercially available Genotype MTBDRplus molecular assay was compared to conventional methods including AFB smear, culture and drug susceptibility testing (DST) using both an absolute concentration method on Lowenstein-Jensen media and broth-based method using the MGIT 960 system. Sputum specimens were obtained from TB suspects in the country of Georgia who received care through the National TB Program. Results Among 500 AFB smear-positive sputum specimens, 458 (91.6%) had both a positive sputum culture for Mycobacterium tuberculosis and a valid MTBDRplus assay result. The MTBDRplus assay detected isoniazid (INH) resistance directly from the sputum specimen in 159 (89.8%) of 177 specimens and MDR-TB in 109 (95.6%) of 114 specimens compared to conventional methods. There was high agreement between the MTBDRplus assay and conventional DST results in detecting MDR-TB (kappa = 0.95, p |
| Databáze: | OpenAIRE |
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