Expression pattern of cochlear microRNAs in the mammalian auditory hindbrain
| Autor: | Mor Bordeynik-Cohen, Constanze Krohs, Karen B. Avraham, Hans Gerd Nothwang, Naama Messika-Gold, Ran Elkon |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cochlear Nucleus Histology Auditory Pathways Evolution Gene regulatory network Prefrontal Cortex Hindbrain Biology Development Cochlear nucleus Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine medicine Auditory system Animals Prefrontal cortex Cochlea Auditory Cortex Mammals Gene Expression Regulation Developmental Regular Article Superior Olivary Complex Cell Biology Mice Inbred C57BL Rhombencephalon MicroRNAs 030104 developmental biology medicine.anatomical_structure Auditory rehabilitation Superior olivary complex Transduction (physiology) Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Cell and Tissue Research |
| ISSN: | 1432-0878 0302-766X |
| Popis: | The auditory system comprises the auditory periphery, engaged in sound transduction and the central auditory system, implicated in auditory information processing and perception. Recently, evidence mounted that the mammalian peripheral and central auditory systems share a number of genes critical for proper development and function. This bears implication for auditory rehabilitation and evolution of the auditory system. To analyze to which extent microRNAs (miRNAs) belong to genes shared between both systems, we characterize the expression pattern of 12 cochlea-abundant miRNAs in the central auditory system. Quantitative real-time PCR (qRT-PCR) demonstrated expression of all 12 genes in the cochlea, the auditory hindbrain and the non-auditory prefrontal cortex (PFC) at embryonic stage (E)16 and postnatal stages (P)0 and P30. Eleven of them showed differences in expression between tissues and nine between the developmental time points. Hierarchical cluster analysis revealed that the temporal expression pattern in the auditory hindbrain was more similar to the PFC than to the cochlea. Spatiotemporal expression analysis by RNA in situ hybridization demonstrated widespread expression throughout the cochlear nucleus complex (CNC) and the superior olivary complex (SOC) during postnatal development. Altogether, our data indicate that miRNAs represent a relevant class of genetic factors functioning across the auditory system. Given the importance of gene regulatory network (GRN) components for development, physiology and evolution, the 12 miRNAs provide promising entry points to gain insights into their molecular underpinnings in the auditory system. Electronic supplementary material The online version of this article (10.1007/s00441-020-03290-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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