Used leucodepletion filters as a source of large quantities of DNA suitable for the study of genetic variations in human populations
| Autor: | Mark Cook, David Philip Lowe, David Briggs, S. L. Farmer, N. Smith, Shirley Jobson, W. J. Alvi-Ali, D. C. Atkinson |
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| Rok vydání: | 2003 |
| Předmět: |
Genetic Markers
Genetic Research Sample (material) Blood Donors Computational biology Cell Separation Biology Polymerase Chain Reaction law.invention Waste product chemistry.chemical_compound law Genetic variation Leukocytes Humans Polymerase chain reaction Genetics Genetic Variation Hematology DNA Sequence Analysis DNA DNA extraction genomic DNA chemistry Genetic marker Filtration |
| Zdroj: | Transfusion medicine (Oxford, England). 13(2) |
| ISSN: | 0958-7578 |
| Popis: | summary. A simple technique for developing large control panels with large quantities of DNA suitable for studies in population genetics was established. Background and objectives Both a lack of suitable controls and insufficient quantities of DNA for repeated analysis of the same control group often hamper the investigation of genetic markers for disease. Materials and methods Using a waste product from routine blood donation, we describe a simple method that allows the investigator to extract large amounts of DNA. Results A mean of 1520 µg of DNA per sample was obtained. The DNA obtained remains suitable for polymerase chain reaction and sequencing techniques after 2 years of storage at both 4 °C and −40 °C. Conclusion This technique allows the development of a large panel of controls with sufficient quantities of genomic DNA for thousands of tests. |
| Databáze: | OpenAIRE |
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