Comparative Proteomics Analysis Reveals L-Arginine Activates Ethanol Degradation Pathways in HepG2 Cells
| Autor: | Qiwen Fan, Zhichang Wang, Xingya Yang, Changqing Chen, Xiuzhi Li, Jun Hu, Baisheng Long, Joelal Achmadi, Xiaozhen Guo, Guokai Yan, Retno Lestari, Xianghua Yan, Agung Purnomoadi, Lu Liu, Jie Yu |
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| Rok vydání: | 2016 |
| Předmět: |
Proteomics
0301 basic medicine Spectrometry Mass Electrospray Ionization Proteome Arginine Blotting Western Biology Aldehyde Dehydrogenase 1 Family Article 03 medical and health sciences Humans Ethanol metabolism Chromatography High Pressure Liquid Alcohol dehydrogenase chemistry.chemical_classification Multidisciplinary Ethanol Aldehyde Dehydrogenase Mitochondrial Alcohol Dehydrogenase Retinal Dehydrogenase Hep G2 Cells Aldehyde Dehydrogenase Up-Regulation Amino acid Blot 030104 developmental biology Enzyme chemistry Biochemistry biology.protein Metabolic Networks and Pathways |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep23340 |
| Popis: | L-Arginine (Arg) is a versatile amino acid that plays crucial roles in a wide range of physiological and pathological processes. In this study, to investigate the alteration induced by Arg supplementation in proteome scale, isobaric tags for relative and absolute quantification (iTRAQ) based proteomic approach was employed to comparatively characterize the differentially expressed proteins between Arg deprivation (Ctrl) and Arg supplementation (+Arg) treated human liver hepatocellular carcinoma (HepG2) cells. A total of 21 proteins were identified as differentially expressed proteins and these 21 proteins were all up-regulated by Arg supplementation. Six amino acid metabolism-related proteins, mostly metabolic enzymes, showed differential expressions. Intriguingly, Ingenuity Pathway Analysis (IPA) based pathway analysis suggested that the three ethanol degradation pathways were significantly altered between Ctrl and +Arg. Western blotting and enzymatic activity assays validated that the key enzymes ADH1C, ALDH1A1 and ALDH2, which are mainly involved in ethanol degradation pathways, were highly differentially expressed and activated between Ctrl and +Arg in HepG2 cells. Furthermore, 10 mM Arg significantly attenuated the cytotoxicity induced by 100 mM ethanol treatment (P |
| Databáze: | OpenAIRE |
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